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Figure 2 | Virology Journal

Figure 2

From: Depletion of polycistronic transcripts using short interfering RNAs: cDNA synthesis method affects levels of non-targeted genes determined by quantitative PCR

Figure 2

RT-PCR quantification of HPV16 mRNA depletion in E7-siRNA treated cells. The top row shows levels of A) E7 and B) E6 24 hours after treatment of CaSki with E7-targeting siRNAs. The bottom row shows levels of C) E7, D) E6 and E) E2 at 24, 48 and 96 hours after treatment of W12Ser4p86 with E7-141 siRNAs. Each pair of bars shows levels detected in the same cells using cDNA generated by oligo-dT primers only (red) or oligo-dT and random hexamer primers (green). Error bars show the standard deviations, based on three independent experiments. NTC = non-targeting control.

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Virology Journal

ISSN: 1743-422X