Figure 4

ATO and CoTx disruption of PML NBs induces EBV lytic protein expression. A) Sections from tumor explants from each of the 4 treatment groups were stained for PML (green). Nuclei were stained with DAPI (blue) (original magnification of 400X). B) Zta positive nuclei and total CNE1 < BX1 > nuclei were counted in random fields comprising multiple sections from each treatment group (Figure 3C). C) RNA was isolated from tumor explant tissue samples and levels of the EBV early protein BMRF1 gene product were assessed by reverse transcriptase real-time PCR.