Figure 2

Overexpression of hnRNP Q does not affect the levels and the nucleocytoplasmic distribution of Rev-dependent reporter mRNAs. A) Schematic organization of the Rev-dependent reporter construct. The 5′-LTR directs transcription of the Ds Red reporter gene. The reporter mRNA contains Rev-dependent regulatory elements (i.e. gag-derived instability elements and a Rev-response element (RRE)). Primers for qRT-PCR analyses bind within the RRE region. B) Total reporter RNA levels were investigated after expression of hnRNP Q-CYN or the CYN control in the presence or absence of Rev. Overexpression of hnRNP Q did not change total levels of reporter mRNA independent of Rev expression. C) Reporter RNA levels were also analyzed after separation of cellular lysates into cytoplasmic and nuclear fractions. Overexpression of hnRNP Q did not enhance export of reporter mRNAs. H2A and GAPDH were used to evaluate purity of cytoplasmic and nuclear fractions, respectively. Bars represent the means of at least 3 independent experiments and standard deviations are indicated. p-values: n.s. = not significant (p > 0.05).