Table 1 DNA sequences of the primers used in this study, their target genes/proteins and positions
Virus | Gene/protein target | Primer sequence | Position | Reference | |
|---|---|---|---|---|---|
1. | Dengue | Structural polyprotein | D1; (5′-TCA ATA TGC TGA AAC GCG CGA GAA ACC G-3′) | 38-65 | [28] |
“ | “ | D2; (5′-TTG CAC CAA CAG TCA ATG TCT TCA GGT TC-3′) | 455-483 | ||
2. | Alphavirus | NSP4 | VIR 2052 F;(5′-TGG CGC TAT GAT GAA ATC TGG AAT GTT-3′) | 6971–6997 | [29] |
“ | “ | VIR 2052R; (5′-TAC GAT GTT GTC GTC GCC GAT GAA-3′) | 7086–7109 | ||
3. | Flavivirus | NS5 | FU 1; (5′- TAC AAC ATG ATG GGA AAG AGA GAG AA-3′) | 9007-9032 | [30] |
“ | “ | CFD2; (5′- GTG TCC CAG CCG GCG GTG TCA TCA GC-3′) | 9308-9283 | ||
4. | bunyavirus | nucleocapsid protein | BCS82C; (5′-ATG ACT GAG TTG GAG TTT CAT GAT GTC GC-3′) | 86-114 | [31] |
“ | “ | BCS332V; (5′-TGT TCC TGT TGC CAG GAA AAT-3′) | 309-329 | ||
5. | Ndumu | envelope (E1) gene | ND 124 F; (5′-CAC CCT AAA AGT GAC GTT-3′) | 124-141 | [30] |
“ | “ | ND 632R; (5′- ATT GCA GAT GGG ATA CCG-3′) | 615-632 | ||
6. | West Nile | helicase | WN1315F; (5′-GCC AA TTT GCC TGC TCT AC-3′) | 1315-1333 | [32] |
“ | “ | WN1824R; (5′-CCA TCT TCA CTC TAC ACT TC-3′) | 1824-1843 | ||
7. | Babanki | E1 envelope glycoprotein | Bab 3368 F; (5′- CAG CAG ATT GCG CGA CTG ACC-3′) | 3368-3388 | [30] |
“ | “ | Bab 4203R; (5′- GCT CAC GAT ATG GTC AGC AGG-3′) | 4184-4203 | ||
8. | Batai | Polyprotein M segment | BATAIM3F; (5′-CCTGGGGAAGCATTGTGATTACT-3′) | 852-874 | [33] |
“ | “ | BATAIM3R; (5′-CTAGCCAGCGACTCTTGCCTTCC-3′) | 2084-2103 | ||
9. | Sindbis | Non Structural Protein | SINV1; (5′-TTTAGCGGATCGGACAATTC-3′) | 5194-5213 | [34] |
“ | “ | SINV2; (5′-GCGGTGACGAACTCAGTAG-3′) | 6482-6500 | ||
10. | RVF | Glycoprotein M gene | RVF1;(5′-GAC TAC CAG TCA GCT CAT TAC C-3′) | 777-798 | [34] |
“ | “ | RVF2;(5′-TGT GAA CAA TAG GCA TTG G-3′) | 1309-1327 | ||
11. | Chikungunya/Onyon’gnyon’g | 5′NTR | CHIK3F; (5′-CACACGTAGCCTACCAGTTTC-3′) | 14-112 | [35] |
“ | “ | CHIK3R; (5′-GCTGTCAGCGTCTATGTCCAC-3′) | “ | ||
“ | “ | ONN3F; (5′-GATACACACACGCAGCTTACG-3′) | 11-97 | ||
“ | “ | ONN3R; (5′-TACATACACTGAATCCATGATGGG-3′) | “ | ||
12. | Yellow Fever | polyprotein | CAG; (5′- CGA GTT GCT AGG CAA TAA ACA CAT TTG GA-3) | 43-71 | [36] |
“ | “ | YF7; (5′- AAT GCT CCC TTT CCC AAA TA- 3′) | 1293-1312 |