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Table 1 DNA sequences of the primers used in this study, their target genes/proteins and positions

From: Mosquito-borne arbovirus surveillance at selected sites in diverse ecological zones of Kenya; 2007 – 2012

  Virus Gene/protein target Primer sequence Position Reference
1. Dengue Structural polyprotein D1; (5′-TCA ATA TGC TGA AAC GCG CGA GAA ACC G-3′) 38-65 [28]
  D2; (5′-TTG CAC CAA CAG TCA ATG TCT TCA GGT TC-3′) 455-483  
2. Alphavirus NSP4 VIR 2052 F;(5′-TGG CGC TAT GAT GAA ATC TGG AAT GTT-3′) 6971–6997 [29]
  VIR 2052R; (5′-TAC GAT GTT GTC GTC GCC GAT GAA-3′) 7086–7109  
3. Flavivirus NS5 FU 1; (5′- TAC AAC ATG ATG GGA AAG AGA GAG AA-3′) 9007-9032 [30]
  CFD2; (5′- GTG TCC CAG CCG GCG GTG TCA TCA GC-3′) 9308-9283  
4. bunyavirus nucleocapsid protein BCS82C; (5′-ATG ACT GAG TTG GAG TTT CAT GAT GTC GC-3′) 86-114 [31]
  BCS332V; (5′-TGT TCC TGT TGC CAG GAA AAT-3′) 309-329  
5. Ndumu envelope (E1) gene ND 124 F; (5′-CAC CCT AAA AGT GAC GTT-3′) 124-141 [30]
  ND 632R; (5′- ATT GCA GAT GGG ATA CCG-3′) 615-632  
6. West Nile helicase WN1315F; (5′-GCC AA TTT GCC TGC TCT AC-3′) 1315-1333 [32]
  WN1824R; (5′-CCA TCT TCA CTC TAC ACT TC-3′) 1824-1843  
7. Babanki E1 envelope glycoprotein Bab 3368 F; (5′- CAG CAG ATT GCG CGA CTG ACC-3′) 3368-3388 [30]
  Bab 4203R; (5′- GCT CAC GAT ATG GTC AGC AGG-3′) 4184-4203  
8. Batai Polyprotein M segment BATAIM3F; (5′-CCTGGGGAAGCATTGTGATTACT-3′) 852-874 [33]
  BATAIM3R; (5′-CTAGCCAGCGACTCTTGCCTTCC-3′) 2084-2103  
9. Sindbis Non Structural Protein SINV1; (5′-TTTAGCGGATCGGACAATTC-3′) 5194-5213 [34]
  SINV2; (5′-GCGGTGACGAACTCAGTAG-3′) 6482-6500  
10. RVF Glycoprotein M gene RVF1;(5′-GAC TAC CAG TCA GCT CAT TAC C-3′) 777-798 [34]
  RVF2;(5′-TGT GAA CAA TAG GCA TTG G-3′) 1309-1327  
11. Chikungunya/Onyon’gnyon’g 5′NTR CHIK3F; (5′-CACACGTAGCCTACCAGTTTC-3′) 14-112 [35]
  CHIK3R; (5′-GCTGTCAGCGTCTATGTCCAC-3′)  
  ONN3F; (5′-GATACACACACGCAGCTTACG-3′) 11-97  
  ONN3R; (5′-TACATACACTGAATCCATGATGGG-3′)  
12. Yellow Fever polyprotein CAG; (5′- CGA GTT GCT AGG CAA TAA ACA CAT TTG GA-3) 43-71 [36]
  YF7; (5′- AAT GCT CCC TTT CCC AAA TA- 3′) 1293-1312