Figure 5
From: Aquareovirus protein VP6 colocalizes with NS80 protein in infected and transfected cells
Subcellular colocalization of the VP6 and NS80 proteins in cotransfected cells. IF microscopy of Vero cells cotransfected with plasmids pCI-NS80 and pEGFP-VP6 (A), pCI-VP6 (B), pEGFP-C1 (C) for 20 h. The intracellular localizations of NS80 and VP6 were detected by immunostaining with rabbit anti-NS80 followed by Alexa Fluor® 488 donkey anti-rabbit IgG (Green, B) or Alexa Fluor® 568 donkey anti-mouse IgG(red, A and C), and mouse anti-VP6 polyclonal antibodies followed by Alexa Fluor® 568 donkey anti-mouse IgG (red, B). The nuclei were visualized by counterstaining with Hoechst (blue).