Figure 3

ZASC1^−/−mice have increased LK cell compartments in the bone marrow. A.) FACS plots gating hematopoietic progenitor populations in the bone marrow. Antibodies used: α-c-kit, α-sca-1, α-CD105, α-CD150, and lineage markers (α-CD11b, α-Gr-1, α-Ter119, α-CD3, α-Ly6C, α-CD19, α-CD11c). LK (Lin^- Sca-1^- C-kit⁺), LSK (Lin^- Sca-1⁺ C-kit⁺), MPP (multipotent progenitor). and HSC (hematopoietic stem cell) populations within the bone marrow were gated as labeled in the top left box. B.) Graphical representation of the LK, LSK and HSC populations from multiple litters. N=3 mice per group. Mean average values. Error bars indicate SD. Two-tailed T-test was used to measure statistically significant differences between ZASC1^+/+ and ZASC1^−/− populations. Significant p-value is indicated.