Comparison of LFI test for tissue suspensions and lesion swabs. Tissue suspensions and flocked swabs over lesions collected from 4 experimentally inoculated pigs with A22 Iraq. Samples (a. tissue suspension, b. lesion swab sample, 50 μl) were mixed with 1 μl of biotinylated F66 A22-14 and 6 μl of colloidal gold conjugated F21-140SO in the running buffer. The mixture (100 μl) was applied to the gRAD. After 10 minutes, the result was recorded.