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Figure 3 | Virology Journal

Figure 3

From: A monoclonal antibody against lymphocyte function-associated antigen-1 decreases HIV-1 replication by inducing the secretion of an antiviral soluble factor

Figure 3

LFA-1 MAb does not inhibit CTL killing of activated uninfected CD4+ target cells. CD4-depleted PBMC effector cells from ten HIV-1 negative and ten HIV positive subjects were co-cultured at a ratio of 25:1 with activated uninfected CFSE labeled CD4+ target cells and cytotoxicity was determined using flow cytometry to differentiate 7AAD + (dead) and 7AAD- (live) target cells. Effector cells from HIV positive subjects were more cytotoxic (p = 0.0115 for PBS, p = 0.0185 for LFA-1 MAb, Mann Whitney test) but the degree of cytotoxicity did not decrease significantly in the presence of LFA-1 MAb for cells from either HIV-1 positive or HIV-1 negative subjects (p = 0.879 for HIV-1 negative, p = 0.5286 for HIV-1 positive, paired t test).

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Virology Journal

ISSN: 1743-422X