Table 2 The amino acid substitutions in the HA2 and character of Stachyflin-resistant (R) virus clones
From: Antiviral activity of stachyflin on influenza A viruses of different hemagglutinin subtypes
Virus | EC50(μM) | Amino acid position in HA2a | ⊿pHc | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
37 | 51 | 85 | 91 | 98 | 107 | 110 | ||||
WSN | Wild type | 0.02 | D | K | D | I | L | T | F | 0.0 |
R1 | >6.50 | N | -b | - | - | - | - | - | 0.3 | |
R2 | >6.50 | - | R | - | - | - | - | - | −0.3 | |
R3 | >6.50 | - | - | H | - | - | - | - | 0.0 | |
R4 | >6.50 | - | - | - | - | - | I | - | 0.2 | |
R5 | >6.50 | - | - | - | - | V | - | - | −0.2 | |
R6 | >6.50 | - | - | - | F | - | - | - | 0.0 | |
PR8 | Wild type | 0.49 | D | K | D | I | L | T | F | N.D.d |
R1 | >6.50 | - | - | - | - | S | - | - | N.D. | |
R2 | >6.50 | - | - | - | - | - | - | S | N.D | |
Ibaraki | Wild type | 0.17 | D | K | D | I | L | T | F | N.D |
R1 | >6.50 | - | R | - | - | - | - | - | N.D. | |
Taiwan | Wild type | 0.44 | D | K | D | I | L | T | F | N.D. |
R1 | >6.50 | - | R | - | - | - | - | - | N.D. | |