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Figure 1 | Virology Journal

Figure 1

From: Pepino mosaic virus genotype shift in North America and development of a loop-mediated isothermal amplification for rapid genotype identification

Figure 1

Comparative analysis of a genotype-specific detection of Pepino mosaic virus isolates using RT-LAMP and RT-PCR. The upper panel (A) was for genotype US1 isolates. The lower panel (B) was for genotype CH2 isolates. Lane M: PCR marker; Lane 1 was a positive control in a mixed infection with two genotypes of PepMV (US1 and CH2); Lanes 2–10 were 9 field samples infected with PepMV; Lane 11 was a health tomato (negative) control; Lane 12 was a non-template (blank) control. PepMV isolates in Lanes 2, 3, 9 and 10 were typed as US1. The isolates in Lanes 4–8 were shown to be CH2 type. Note, the faint products in lanes 7–8 by RT-PCR, were clearly visible by RT-LAMP.

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Virology Journal

ISSN: 1743-422X