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Figure 5 | Virology Journal

Figure 5

From: Synthetic immunotherapy induces HIV virus specific Th1 cytotoxic response and death of an HIV-1 infected human cell line through classic complement activation

Figure 5

Complement mediated cell lysis of HIV-1 infected human cells measured as LDH release. Heat inactivated sera from HIV-v and NRP-v immunised mice were diluted 1/100 in PBS, combined with rabbit complement and added to cultures of T1 cells infected with HIV-1 field isolate UG-29-029 (clade A) or laboratory strain IIIB. The percentage of cell lysis was defined between the level of LDH release of non-infected T1 cells in the presence of complement without sera (0%) and the LDH released after lysing the same cells with Triton X-100 (100%). A positive response, indicated by an asterisk (*), was defined as an increment of lysis of at least 50% over that of HIV-1 infected cells in the presence of control (NRP-v) sera and with a statistical significance of p < 0.05. Represented are the averages of percentage cell lysis ± SEM.

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Virology Journal

ISSN: 1743-422X