Figure 1

Panel A: Hypothetical model illustrating the antigenic structure of gD and demonstrating the clustering of antigenic sites into seven groups, as defined by locations of amino acids bound by various monoclonal antibodies. Disulphide bonds location defined by braces. Diagram adapted with permission from Nicola et al, 1998 [22]. Of particular relevance to this study are the locations of sites VII (amino acid residues 11–19), which is bound by antibody 1D3, and site Ib, a discontinuous epitope that includes residues 222 to 252 that is bound by antibody DL11. Panel B: Diagram showing interface between N-terminal amino acids of gD and HveA and the approximate residues (blue) bound by monoclonal antibody 1D3 and, by inference, 1D3 scFv (adapted with permission from Connolly et al, 2003 [19].