High hepatitis B virus load is associated with hepatocellular carcinomas development in Chinese chronic hepatitis B patients: a case control study
© Zhou et al; BioMed Central Ltd. 2012
Received: 29 June 2011
Accepted: 13 January 2012
Published: 13 January 2012
Persistent hepatitis B virus (HBV) infection is a risk factor for hepatocellular carcinoma (HCC) development. This study aimed to clarify whether the high HBV DNA level is associated with HCC development by comparing HBV DNA levels between HBV infected patients with and without HCC.
There were 78 male and 12 female patients in each group and there was no statistical difference between these two group patients' average ages. The HBV DNA level in the HCC patients was 4.73 ± 1.71 Log10 IU/ml while 3.90 ± 2.01 Log10 IU/ml in non-HCC patients (P < 0.01). The HBeAg positive rate was 42.2% (38/90) in the HCC group while 13.3% (12/90) in the non-HCC group (P < 0.001). Compared with patients with HBV DNA level of < 3 Log10 IU/ml, the patients with level of 3 to < 4, 4 to < 5, 5 to < 6, or ≥ 6 Log10 IU/ml had the odds ratio for HCC of 1.380 (95% CI, 0.544-3.499), 3.671 (95% CI, 1.363-9.886), 5.303 (95% CI, 1.847-15.277) or 3.030 (95% CI, 1.143-8.036), respectively.
HBV-related HCC patients had higher HBV DNA level than non-HCC counterparts. Our findings imply that active HBV replication is associated with the HCC development.
Chronic hepatitis B virus (HBV) infection is a serious public health problem worldwide since more than 350 million people are chronic carriers [1–4]. Persistent HBV infection is a risk factor for the development of hepatocellular carcinoma (HCC). The viral factors, such as viral genotype and mutants, have been shown to be associated with the pathogenesis of HCC [5–7]. Recent studies show that HBV load may also be associated with the HCC development [8–15] because the HBV DNA level in HCC patients was higher than that in non-HCC patients [13–15]. However, other studies showed that the viral load in HBV-associated HCC patients was not higher than that in the patients without HCC [16, 17].
HBV infection is still highly endemic in China although the prevalence of hepatitis B surface antigen (HBsAg) has been reduced from 10% to 7.18% after the hepatitis B vaccine has been widely used in infants [18, 19]. The age-standardized incidence of HCC in China is 58 per 100 000 persons for men and 22 per 100 000 persons for women, which is high in the world . However, the comparison of HBV DNA level between HCC and non-HCC patients in China has been less studied . In view of high HBV endemicity and HCC incidence in China, we performed this study to compare serum HBV DNA level between chronic hepatitis B (CHB) patients with HCC and the patients with HBV infection alone to clarify whether the high HBV level is a risk for HCC development.
Characteristics of HCC and non-HCC patients
HCC (n = 90)
non-HCC (n = 90)
52.53 ± 10.27
52.53 ± 10.23
Total Protein (g/L)
68.2 ± 6.8
65.5 ± 8.1
38.5 ± 5.0
38.9 ± 6.4
29.7 ± 6.5
26.6 ± 5.6
Total Bilirubin (μmol/L)
Direct Bilirubin (μmol/L)
The percentage of patients with abnormal serum biochemical parameters
HCC (n = 90)
non-HCC (n = 90)
Comparison of HCC and non-HCC patients' HBV DNA level
Comparison of the HCC and non-HCC patients' number and viral load stratified by ages
Patients number (%)
viral load (Log10 IU/ml, mean ± SD)
4.73 ± 1.71
3.90 ± 2.01
4.48 ± 1.80
3.56 ± 1.35
5.20 ± 1.52
3.98 ± 2.21
4.20 ± 1.85
4.20 ± 2.09
5.51 ± 0.94
3.61 ± 2.46
Odds ratios for the association between HCC and various HBV load
HBV load (IU/ml)
OR (95% CI)
105 - < 106
104 - < 105
103 - < 104
We quantitatively measured serum HBV load in 90 HBV infected patients with HCC and in 90 age- and gender-matched patients with HBV infection alone and found that HCC patients had higher serum HBV DNA level than patients without HCC, especially in the age range of 50-59 years or below 40 years. The data in the present study indicate that the patients with higher HBV level have more risk to develop HCC.
There were a few retrospective studies in which the authors compared HCC and non-HCC patients' HBV DNA level to clarify the role of HBV load in HCC development. Some authors stated the level in HCC patients was higher than that in non-HCC patients [14, 15] and > 104  or > 105  copies/ml HBV DNA level was supposed to be an independent risk factor of HCC development. As an indicator of active replication of HBV, the higher HBeAg positive rate is usually associated with an increased risk of HCC . Our findings were similar to these previously reported results. However, in Tsai et al's paper, there existed no significant difference in HCC and non-HCC patients' HBV DNA level . Another study performed in India showed that the HBV DNA load in HCC patients was lower than that of HBV-related chronic liver disease patients . However, in these two studies, the HCC group patients were both obviously older than the non-HCC group patients (53.7 ± 12.7 vs. 32.1 ± 10.0 years , 53.6 ± 13.2 vs. 44.2 ± 14.7 years ). The evolution and the viral load of HBV infection may be influenced by age at acquisition. In the present study, although it is difficult to determine duration of the infection for each patient, we considered that most patients in our study had been infected with HBV in their infantile or childhood periods as the majority of chronic HBV infection in China is due to the perinatal infections . Thus, the comparable patients' ages in the HCC and non-HCC groups may represent the similar infection durations in the two groups. Since HCC is a long-term outcome of chronic HBV infection and HBV DNA level is usually higher in younger CHB patients than in older ones , the matching of the patients' age is essential in the comparison of HBV DNA level in patients with or without HCC.
Large number chronic HBsAg carriers in Taiwan were followed up for a long period and the prospective data showed an increased risk for cirrhosis and for HCC with increasing level of HBV DNA [24, 25]. Several studies have suggested an increased risk of HCC with high HBV DNA level (> 106 copies/ml , 104-107 copies/ml  or > 105 copies/ml ). In our study, the data demonstrated that the patients with higher than 104 IU/ml HBV DNA level had more risk to be diagnosed as HCC, which was similar to the previous prospective reports [25–29]. The commonly used quantitative units of HBV DNA level in papers are IU/ml and copies/ml. In the current WHO HBV standard and consensus, one IU is approximately equivalent to five genome equivalents (copies) . The viral load of 104 IU/ml identified in this study is approximately equal to 5 × 104 copies/ml.
In summary, our findings demonstrated that higher serum HBV load in HCC patients than non-HCC counterparts, especially in the age range of both 50-60 years and below 40 years. Our results suggest that patients with higher than 104 IU/ml HBV DNA level are associated with more risk to develop HCC.
Patients and study design
A total of 388 CHB patients were admitted to Nanjing Drum Tower Hospital between October 2009 and June 2010. All patients were positive for HBsAg for at least 6 months. One group included 120 CHB patients who were diagnosed with HCC for the first time. The diagnosis of HCC was made by pathological findings or a combination of elevated α-fetoprotein (> 400 ng/ml) and typical appearance on at least two radiological imaging techniques, including ultrasonography, computerized tomography, magnetic resonance imaging, or hepatic angiography with lipiodol. The other group was 268 CHB patients without HCC. The patients with hepatitis C virus (HCV) or HIV co-infection, primary biliary cirrhosis, autoimmune hepatitis, significant alcohol intake or Wilson's disease were excluded. After we excluded the patients who received any antiviral treatment before, these two group patients were matched for age (fewer than 2 years difference) and gender respectively. As a result, there were 90 patients in each group. All study participants provided informed written consent. All the experiments were approved by the Ethics Committee of Nanjing Drum Tower Hospital, Nanjing University Medical School, in accordance with guidelines of the Nation Health and Medical Research Council of China.
A blood sample was collected from each subject and the serum was separated by centrifugation at room temperature. The serum was stored in a sterile tube at -20°C until use. For HCC patients, serum samples were collected before surgical resection or interventional therapy was performed.
Viral markers assays
HBsAg and hepatitis B e antigen (HBeAg) were tested by commercial enzyme-linked immunosorbent assays (ELISA) (Huakang Biotechnology, Shenzhen, China). Commercial ELISA kit for anti-HCV was obtained from Huakang (Huakang Biotechnology, Shenzhen, China) and anti-HIV ELISA kit was from Lizhu (Lizhu Biotechnology, Zhuhai, China).
Quantitative assay of HBV DNA
Serum HBV DNA was quantified with a commercial fluorescent real-time PCR assay (DaAn Gene, Guangzhou, China) . Serum DNA was extracted from 100 μl serum by boiling in DNA extract buffer (NaOH, Tris-HCl, TritonX-100, NP40, chelex-100, EDTA) and was dissolved in 20 μl DNA solution buffer. Afterwards, 2 μl DNA solution was subjected to PCR. The PCR program consisted of 93°C for 2 min, 10 cycles of 93°C for 45 seconds and 55°C for 60 seconds, 30 cycles of 93°C for 30 seconds and 55°C for 45 seconds. The fluorescence signal of the amplicons was detected in every step of 55°C for 45 seconds by 7500 real time PCR system (Applied Biosystems, Singapore). The lower detection limit of this assay was 103 IU/ml with a linear range of up to 107 IU/ml. The known amounts of 103, 104, 105, 106, 107 IU/ml HBV DNA were used as a control. Strict precautions were taken to avoid potential contamination.
Serum HBV DNA level was log10-transformed for analysis. Normal distribution data were expressed as mean ± standard deviation and non-normal distribution data were given as median with range. All the HBV DNA levels below the detection limit (3 log10 IU/ml) were defined as non-measured values and they were arbitrarily estimated as 1.5 log10 IU/ml in analysis for the reason of estimated continuous distribution of the HBV DNA levels. The differences in liver function and serum DNA level between HCC and non-HCC patients were evaluated by Pearson's Chi-square test, Student's t test and Mann-Whitney u test where appropriate. All estimates were accompanied by a 95% confidence interval (CI) where appropriate and two-sided P values of fewer than 0.05 were considered statistically significant.
We thank Dr. Decai Yu for collecting some HCC patients' sera. This study was supported by a Special Research Grant (RC2007005) for the Principal Fellow from the Department of Health, Jiangsu Province, China.
- Marcellin P: Hepatitis B and hepatitis C in 2009. Liver Int. 2009, 29 (Suppl 1): 1-8.PubMedView ArticleGoogle Scholar
- Van Damme P, Zanetti AR, Shouval D, Van Herck K: Strategies for global prevention of hepatitis B virus infection. Adv Exp Med Biol. 2010, 659: 175-188. 10.1007/978-1-4419-0981-7_14.PubMedView ArticleGoogle Scholar
- Te HS, Jensen DM: Epidemiology of hepatitis B and C viruses: a global overview. Clin Liver Dis. 2010, 14: 1-21. 10.1016/j.cld.2009.11.009.PubMedView ArticleGoogle Scholar
- Chinese Society of Hepatology. Chinese Medical Association and Chinese Society of Infectious Diseases, Chinese Medical Association: Guideline on prevention and treatment of chronic hepatitis B in China (2005). Chin Med J (Engl). 2007, 120: 2159-2173.Google Scholar
- Lim SG, Mohammed R, Yuen MF, Kao JH: Prevention of hepatocellular carcinoma in hepatitis B virus infection. J Gastroenterol Hepatol. 2009, 24: 1352-1357. 10.1111/j.1440-1746.2009.05985.x.PubMedView ArticleGoogle Scholar
- Asia-Pacific Working Party on Prevention of Hepatocellular Carcinoma: Prevention of hepatocellular carcinoma in the Asia-Pacific region: consensus statements. J Gastroenterol Hepatol. 2010, 25: 657-663.View ArticleGoogle Scholar
- Chen CJ, Chen DS: Interaction of hepatitis B virus, chemical carcinogen, and genetic susceptibility: multistage hepatocarcinogenesis with multifactorial etiology. Hepatology. 2002, 36: 1046-1049. 10.1053/jhep.2002.37084.PubMedView ArticleGoogle Scholar
- Tsai WL, Chung RT: Viral hepatocarcinogenesis. Oncogene. 2010, 29: 2309-2324. 10.1038/onc.2010.36.PubMedPubMed CentralView ArticleGoogle Scholar
- Ohata K, Hamasaki K, Toriyama K, Ishikawa H, Nakao K, Eguchi K: High viral load is a risk factor for hepatocellular carcinoma in patients with chronic hepatitis B virus infection. J Gastroenterol Hepatol. 2004, 19: 670-675. 10.1111/j.1440-1746.2004.03360.x.PubMedView ArticleGoogle Scholar
- Harris RA, Chen G, Lin WY, Shen FM, London WT, Evans AA: Spontaneous clearance of high-titer serum HBV DNA and risk of hepatocellular carcinoma in a Chinese population. Cancer Causes Control. 2003, 14: 995-1000.PubMedView ArticleGoogle Scholar
- Ohkubo K, Kato Y, Ichikawa T, Kajiya Y, Takeda Y, Higashi S, Hamasaki K, Nakao K, Nakata K, Eguchi K: Viral load is a significant prognostic factor for hepatitis B virus-associated hepatocellular carcinoma. Cancer. 2002, 94: 2663-2668. 10.1002/cncr.10557.PubMedView ArticleGoogle Scholar
- Chu CJ, Hussain M, Lok AS: Quantitative serum HBV DNA levels during different stages of chronic hepatitis B infection. Hepatology. 2002, 36: 1408-1415.PubMedView ArticleGoogle Scholar
- Liu CJ, Chen BF, Chen PJ, Lai MY, Huang WL, Kao JH, Chen DS: Role of hepatitis B virus precore/core promoter mutations and serum viral load on noncirrhotic hepatocellular carcinoma: a case-control study. J Infect Dis. 2006, 194: 594-599. 10.1086/505883.PubMedView ArticleGoogle Scholar
- Fung J, Yuen MF, Yuen JC, Wong DK, Lai CL: Low serum HBV DNA levels and development of hepatocellular carcinoma in patients with chronic hepatitis B: a case-control study. Aliment Pharmacol Ther. 2007, 26: 377-382. 10.1111/j.1365-2036.2007.03390.x.PubMedView ArticleGoogle Scholar
- Xu J, Lin Y, Wang YP, Chen YX, Shi B, Lu J, Xie WF: Hepatitis B virus DNA in patients with hepatitis B-related liver cirrhosis with or without hepatocellular carcinomas: a matched case-control study. J Dig Dis. 2009, 10: 138-144. 10.1111/j.1751-2980.2009.00376.x.PubMedView ArticleGoogle Scholar
- Tsai FC, Liu CJ, Chen CL, Chen PJ, Lai MY, Kao JH, Chen DS: Lower serum viral loads in young patients with hepatitis-B-virus-related hepatocellular carcinoma. J Viral Hepat. 2007, 14: 153-160. 10.1111/j.1365-2893.2006.00780.x.PubMedView ArticleGoogle Scholar
- Asim M, Malik A, Sarma MP, Polipalli SK, Begum N, Ahmad I, Khan LA, Husain SA, Akhtar N, Husain S, Thayumanavan L, Singla R, Kar P: Hepatitis B virus BCP, Precore/core, × gene mutations/genotypes and the risk of hepatocellular carcinoma in India. J Med Virol. 2010, 82: 1115-1125. 10.1002/jmv.21774.PubMedView ArticleGoogle Scholar
- Zhou YH, Wu C, Zhuang H: Vaccination against hepatitis B: the Chinese experience. Chin Med J (Engl). 2009, 122: 98-102.Google Scholar
- Liang X, Bi S, Yang W, Wang L, Cui G, Cui F, Zhang Y, Liu J, Gong X, Chen Y, Wang F, Zheng H, Guo J, Jia Z, Ma J, Wang H, Luo H, Li L, Jin S, Hadler SC, Wang Y: Epidemiological serosurvey of hepatitis B in China-declining HBV prevalence due to hepatitis B vaccination. Vaccine. 2009, 27: 6550-6557. 10.1016/j.vaccine.2009.08.048.PubMedView ArticleGoogle Scholar
- Yuen MF, Hou JL, Chutaputti A: Hepatocellular carcinoma in the Asia pacific region. J Gastroenterol Hepatol. 2009, 24: 346-353. 10.1111/j.1440-1746.2009.05784.x.PubMedView ArticleGoogle Scholar
- Yuen MF, Tanaka Y, Shinkai N, Poon RT, But DY, Fong DY, Fung J, Wong DK, Yuen JC, Mizokami M, Lai CL: Risk for hepatocellular carcinoma with respect to hepatitis B virus genotypes B/C, specific mutations of enhancer II/core promoter/precore regions and HBV DNA levels. Gut. 2008, 57: 98-102.PubMedView ArticleGoogle Scholar
- Yang HI, Lu SN, Liaw YF, You SL, Sun CA, Wang LY, Hsiao CK, Chen PJ, Chen DS, Chen CJ: Hepatitis B e antigen and the risk of hepatocellular carcinoma. N Engl J Med. 2002, 347: 168-174. 10.1056/NEJMoa013215.PubMedView ArticleGoogle Scholar
- Yao GB: Importance of perinatal versus horizontal transmission of hepatitis B virus infection in China. Gut. 1996, 38 (Suppl 2): S39-S42. 10.1136/gut.38.Suppl_2.S39.PubMedPubMed CentralView ArticleGoogle Scholar
- Yu MW, Yeh SH, Chen PJ, Liaw YF, Lin CL, Liu CJ, Shih WL, Kao JH, Chen DS, Chen CJ: Hepatitis B virus genotype and DNA level and hepatocellular carcinoma: a prospective study in men. J Natl Cancer Inst. 2005, 97: 265-272. 10.1093/jnci/dji043.PubMedView ArticleGoogle Scholar
- Chen CJ, Yang HI, Su J, Jen CL, You SL, Lu SN, Huang GT, Iloeje UH: Risk of hepatocellular carcinoma across a biological gradient of serum hepatitis B virus DNA level. JAMA. 2006, 295: 65-73. 10.1001/jama.295.1.65.PubMedView ArticleGoogle Scholar
- Liu TT, Fang Y, Xiong H, Chen TY, Ni ZP, Luo JF, Zhao NQ, Shen XZ: A case-control study of the relationship between hepatitis B virus DNA level and risk of hepatocellular carcinoma in Qidong, China. World J Gastroenterol. 2008, 14: 3059-3063. 10.3748/wjg.14.3059.PubMedPubMed CentralView ArticleGoogle Scholar
- Ito K, Arai M, Imazeki F, Yonemitsu Y, Bekku D, Kanda T, Fujiwara K, Fukai K, Sato K, Itoga S, Nomura F, Yokosuka O: Risk of hepatocellular carcinoma in patients with chronic hepatitis B virus infection. Scand J Gastroenterol. 2010, 45: 243-249. 10.3109/00365520903450113.PubMedView ArticleGoogle Scholar
- Chan HL, Wong VW, Wong GL, Chim AM, Lai LH, Sung JJ: Evaluation of impact of serial hepatitis B virus DNA levels on development of hepatocellular carcinoma. J Clin Microbiol. 2009, 47: 1830-1836. 10.1128/JCM.00029-09.PubMedPubMed CentralView ArticleGoogle Scholar
- Chen G, Lin W, Shen F, Iloeje UH, London WT, Evans AA: Past HBV viral load as predictor of mortality and morbidity from HCC and chronic liver disease in a prospective study. Am J Gastroenterol. 2006, 101: 1797-1803. 10.1111/j.1572-0241.2006.00647.x.PubMedView ArticleGoogle Scholar
- Bowden DS, Thompson AJ: New developments in HBV molecular diagnostics and quantitative serology. Hepatol Int. 2008, 2: 3-11.PubMedPubMed CentralView ArticleGoogle Scholar
- Liu BM, Li T, Xu J, Li XG, Dong JP, Yan P, Yang JX, Yan L, Gao ZY, Li WP, Sun XW, Wang YH, Jiao XJ, Hou CS, Zhuang H: Characterization of potential antiviral resistance mutations in hepatitis B virus reverse transcriptase sequences in treatment-naive Chinese patients. Antiviral Res. 2010, 85: 512-519. 10.1016/j.antiviral.2009.12.006.PubMedView ArticleGoogle Scholar
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