Phenotypic and genotypic variations within a single bacteriophage species
- Pieter-Jan Ceyssens†1,
- Thea Glonti†2,
- ndrew M Kropinski3, 4,
- Rob Lavigne1Email author,
- Nina Chanishvili2,
- Leonid Kulakov5,
- Nino Lashkhi2,
- Marina Tediashvili2 and
- Maya Merabishvili2, 6
© Ceyssens et al; licensee BioMed Central Ltd. 2011
Received: 19 January 2011
Accepted: 23 March 2011
Published: 23 March 2011
Although horizontal gene transfer plays a pivotal role in bacteriophage evolution, many lytic phage genomes are clearly shaped by vertical evolution. We investigated the influence of minor genomic deletions and insertions on various phage-related phenotypic and serological properties.
We collected ten different isolates of Pseudomonas aeruginosa bacteriophage ϕKMV. All sequenced genomes (42-43 kb, long direct terminal repeats) are nearly identical, which intuitively implied strongly similar infections cycles. However, their latent periods vary between 21 and 28 minutes and they are able to lyse between 5 and 58% of a collection of 107 clinical P. aeruginosa strains. We also noted that phages with identical tail structures displayed profound differences in host spectra. Moreover, point mutations in tail and spike proteins were sufficient to evade neutralization by two phage-specific antisera, isolated from rabbits.
Although all analyzed phages are 83-97% identical at the genome level, they display a surprisingly large variation in various phenotypic properties. The small overlap in host spectrum and their ability to readily escape immune defences against a nearly identical phage are promising elements for the application of these phages in phage therapy.
The potential use of lytic bacteriophages as alternative therapeutic agents against antibiotic-resistant bacteria has been widely documented [1–3]. This renewed interest in phage therapy resulted in human clinical trials [4, 5] and a considerable rise of commercial interest . Potential downsides of phage applications, i.e. their relatively narrow host range and emerging bacterial resistance, are often countered by the proposed use of mixtures (cocktails) of phages targeting different hosts and/or host receptors. A popular argument in favour of these cocktails is the sheer abundance of phages in nature (1031 particles worldwide) and the ease of isolating new phages infecting phage-resistant bacteria .
In spite of their ubiquitous character, numerous studies suggest the existence of only a limited number of virulent phage 'types' targeting a specific bacterial species. For example, despite decades of intensive research, only 17 and 13 distinct phage species infecting the model organisms Escherichia coli and Pseudomonas aeruginosa are known [8, 9]. Nearly all newly isolated lytic phages infecting these organisms cluster within existing species and carry genomes which are often over 90% identical at the nucleotide level [10–12]. As this limited global diversity might hamper the composition of truly diverse phage cocktails, we were interested in the impact of these subtle "intraspecies" genomic insertions and deletions on infection-related and serological properties of lytic bacteriophages.
As a model species, we choose the virulent P. aeruginosa phage ϕKMV which resembles the classic coliphage T7 in morphology and overall genome architecture . It was also the first known T7-related phage encoding a single-subunit RNA polymerase gene downstream its DNA metabolism genes instead of in the early genomic region . Up to now, three phages infecting P. aeruginosa (LKD16, ϕKF77 and LUZ19) have been reported to be 83-90% identical to ϕKMV at the nucleotide level [14–16]. In addition, phage LUZ2 was reported to be closely related to ϕKMV based on de novo analysis of structural phage proteins .
Overview of ϕKMV-related bacteriophages included in this study, all infecting P. aeruginos a.
Latent period (min)
Burst size (PFU/cell)
1.6 × 10-8
1.2 × 10-7
1.5 × 10-8
9.2 × 10-9
7.3 × 10-9
2.1 × 10-8
3.9 × 10-9
Using traditional Sanger dideoxy methods for PNM and 454 pyrosequencing for PT2 and PT5, the complete genome sequences of these phages were determined (Table 1, Figure 1). All three phages display high nucleotide similarity (94-97% identity) to ϕKMV, except for a 382 bp region located between ORF3 and 4 (Figure 1), which was earlier associated with a localized single-stranded nick on the non-coding strand in the related phages LUZ19 and ϕKF77 . As ϕKMV is the only phage of this species which has lost this nick, it is most probably a more recent representative of this phage species.
In a final step, the serological relatedness of these phages was studied by using a classical cross neutralization method . Two anti-phage sera (APS) were obtained by a two-step immunization of three Swiss white rabbits with phages PT5 and PNC101. Each rabbit was injected intramuscularly with a 1:1 (vol/vol) mixture of phage (1010 pfu/ml) and adjuvant (Freund's complete adjuvant, Difco). One month later, a secondary immunization was performed by injecting phage (1 ml, 1011 pfu/ml) without adjuvant. After two weeks, blood was taken from the left ventricle of the heart. This blood was allowed to clot, the APS was filtered (0.45 μm pore size) and stored at 4°C. Subsequent neutralization assays with these APS showed significant variation in serological response among the tested phages, as only ϕKMV and PT5 were efficiently neutralized by both antisera (Table 1). Phages LKA1 and LUZ19 were not neutralized to any significant degree by either APS, while the inactivation of LKD16, ϕKF77 and PNM was at least tenfold less efficient than of the phages used for the APS production.
When looking into these results in more detail, some surprising observations were made. One would expect that neutralizing antibodies primarily function through interaction with capsid and/or tail (fibers) proteins of these phages. It is therefore curious that PT2 but not PNM is neutralized by APSPT5, since the structural proteins of PT2 and PNM are all >99.5% identical (Figure 2). Comparing the genomes of these three phages, the only non-silent structural mutations present in PT2 and PT5 in comparison to PNM are found in the tail tubular proteins A (P20-L20, K55-R55, T102-P102, R115-H115) and B (D26-G26, T111-N111, V142-I142, A298-G298, G329-D329, R363-S363, A644-V644, P660-S660, A784-V784). These amino acids substitutions are conserved in ϕKMV, explaining the sensitivity of this phage for APSPT5. A similar finding was observed using the anti-phage serum produced against the non-sequenced phage PNC101. This serum was over tenfold more effective in neutralizing ϕKMV and PT5 than PT2 and PNM, despite 100% identity of their capsid and connector proteins (Table 1, Figure 2). This time, the difference might be correlated to four amino acid substitutions in the spike protein (gp48) of PT2 and PNM.
Although horizontal gene transfer plays a pivotal role in bacteriophage evolution, many lytic phage genomes are clearly shaped by vertical evolution. The high level of genomic conservation (83-97% identity) observed within the 'phiKMV-like viruses' is a common theme among virulent phages infecting the same bacterial host; for example, the genome of Roseophage SIO1 was found to be completely conserved in isolates sampled over many years in various places . Typically, strongly related phages attach to the same receptor and carry out a highly comparable infection cycle. At first glace, this is indeed the case for all analyzed ϕKMV-like viruses. They are all pili-dependent and quickly lyse their host upon the release of up to 166 newly produced particles.
During this study we noticed that small "intraspecies" genomic variations can have essential phenotypic consequences towards the applications of these phages in therapeutic settings. First of all, only limited overlaps in host spectrum exist between isolates with identical tail fibers and/or early genome regions, implying that minor genomic changes can cause a significant shift in infectivity range. Although these differences could be attributed to the evasion of CRISPR repeats , a recent survey did not find a single spacer matching a lytic bacteriophage sequence in 122 clinical P. aeruginosa strains . Alternatively, small point mutations could help in the evasion of host restriction-modification systems . In any case, this illustrates the versatility of a phage genome to evade host defenses.
Moreover, phage therapy seems not be hindered by the adaptive immune system, since minor variations in tail/capsid structures seem sufficient to evade antibody binding. This confirms observations made by Vitiello and colleagues , who reported a long-circulating mutant of phage λ which carried only a single mutation in the major capsid protein. As phages have a virtually unlimited potential of subtly modifying their virion, the potential is present to quickly overcome host immune responses and bacterial resistance during phage therapy.
A.M.K. is supported by a Discovery Grant from the Natural Sciences and Engineering Research Council of Canada. P.J.C. is a postdoctoral fellow supported by the 'Fonds Wetenschappelijk Onderzoek (F.W.O)' of the Flemish Government. This research was performed by members of the 'PhageBiotics' research community, funded by the FWO Vlaanderen (WO.022.09).
- Housby JN, Mann NH: Phage therapy. Drug Discov Today. 2009, 14: 536-540. 10.1016/j.drudis.2009.03.006.View ArticlePubMedGoogle Scholar
- Fischetti VA: Bacteriophage endolysins: a novel anti-infective to control Gram-positive pathogens. Int J Med Microbiol. 2010, 300: 357-362. 10.1016/j.ijmm.2010.04.002.PubMed CentralView ArticlePubMedGoogle Scholar
- Górski A, Miedzybrodzki R, Borysowski J, Weber-Dabrowska B, Lobocka M, Fortuna W, Letkiewicz S, Zimecki M, Filby G: Bacteriophage therapy for the treatment of infections. Curr Opin Investig Drugs. 2009, 10: 766-774.PubMedGoogle Scholar
- Wright A, Hawkins CH, Anggård EE, Harper DR: A controlled clinical trial of a therapeutic bacteriophage preparation in chronic otitis due to antibiotic-resistant Pseudomonas aeruginosa; a preliminary report of efficacy. Clin Otolaryngol. 2009, 34: 349-357. 10.1111/j.1749-4486.2009.01973.x.View ArticlePubMedGoogle Scholar
- Rhoads DD, Wolcott RD, Kuskowski MA, Wolcott BM, Ward LS, Sulakvelidze A: Bacteriophage therapy of venous leg ulcers in humans: results of a phase I safety trial. J Wound Care. 2009, 18: 237-238.View ArticlePubMedGoogle Scholar
- Deresinski S: Bacteriophage therapy: exploiting smaller fleas. Clin Infect Dis. 2009, 48: 1096-1101. 10.1086/597405.View ArticlePubMedGoogle Scholar
- Loc-Carrillo C, Abedon ST: Pros and cons of phage therapy. Bacteriophage. 1: 1-4.Google Scholar
- Casjens SR: Diversity among the tailed-bacteriophages that infect the Enterobacteriaceae. Res Microbiol. 2008, 159: 340-348. 10.1016/j.resmic.2008.04.005.PubMed CentralView ArticlePubMedGoogle Scholar
- Ceyssens PJ, Lavigne R: Bacteriophages of Pseudomonas. Future Microbiol. 2010, 5: 1041-1055. 10.2217/fmb.10.66.View ArticlePubMedGoogle Scholar
- Savalia D, Westblade LF, Goel M, Florens L, Kemp P, Akulenko N, Pavlova O, Padovan JC, Chait BT, Washburn MP, Ackermann HW, Mushegian A, Gabisonia T, Molineux I, Severinov K: Genomic and proteomic analysis of phiEco32, a novel Escherichia coli bacteriophage. J Mol Biol. 2008, 377: 774-789. 10.1016/j.jmb.2007.12.077.PubMed CentralView ArticlePubMedGoogle Scholar
- Angly F, Youle M, Nosrat B, Srinagesh S, Rodriguez-Brito B, McNairnie P, Deyanat-Yazdi G, Breitbart M, Rohwer F: Genomic analysis of multiple Roseophage SIO1 strains. Environ Microbiol. 2009, 11: 2863-2873. 10.1111/j.1462-2920.2009.02021.x.View ArticlePubMedGoogle Scholar
- Ceyssens PJ, Noben JP, Ackermann HW, Verhaegen J, De Vos D, Pirnay JP, Merabishvili M, Vaneechoutte M, Chibeu A, Volckaert G, Lavigne R: Survey of Pseudomonas aeruginosa and its phages: de novo peptide sequencing as a novel tool to assess the diversity of worldwide collected viruses. Environ Microbiol. 2009, 11: 1303-1313. 10.1111/j.1462-2920.2008.01862.x.View ArticlePubMedGoogle Scholar
- Lavigne R, Burkal'tseva MV, Robben J, Sykilinda NN, Kurochkina LP, Jonckx B, Grymonprez B, Krylov VN, Mesyanzhinov VV, Volckaert G: The genome of bacteriophage ϕKMV, a T7-like virus infecting Pseudomonas aeruginosa. Virology. 2003, 312: 49-59. 10.1016/S0042-6822(03)00123-5.View ArticlePubMedGoogle Scholar
- Ceyssens PJ, Lavigne R, Mattheus W, Chibeu A, Hertveldt K, Mast J, Robben J, Volckaert G: Genomic analysis of Pseudomonas aeruginosa phages LKD16 and LKA1: establishment of the ϕKMV subgroup within the T7 supergroup. J Bacteriol. 2006, 188: 6924-6931. 10.1128/JB.00831-06.PubMed CentralView ArticlePubMedGoogle Scholar
- Lammens E, Ceyssens PJ, Voet M, Hertveldt K, Lavigne R, Volckaert G: Representational Difference Analysis (RDA) of bacteriophage genomes. J Microbiol Methods. 2009, 77: 207-213. 10.1016/j.mimet.2009.02.006.View ArticlePubMedGoogle Scholar
- Kulakov LA, Ksenzenko VN, Shlyapnikov MG, Kochetkov VV, Del Casale A, Allen CC, Larkin MJ, Ceyssens PJ, Lavigne R: Genomes of "phiKMV-like viruses" of Pseudomonas aeruginosa contain localized single-strand interruptions. Virology. 2009, 391: 1-4. 10.1016/j.virol.2009.06.024.View ArticlePubMedGoogle Scholar
- Glonti T, Chanishvili N, Taylor PW: Bacteriophage-derived enzyme that depolymerases the alginic acid capsule associated with cystic fibrosis isolates of Pseudomonas aeruginosa. J Appl Microbiol. 2010, 108: 695-702. 10.1111/j.1365-2672.2009.04469.x.View ArticlePubMedGoogle Scholar
- Waddell TE, Franklin K, Mazzocco A, Johnson RP: Preparation and characterization of anti-phage serum. Methods Mol Biol. 2009, 501: 287-292. full_text.View ArticlePubMedGoogle Scholar
- Deveau H, Garneau JE, Moineau S: CRISPR/Cas system and its role in phage-bacteria interactions. Annu Rev Microbiol. 2010, 64: 475-493. 10.1146/annurev.micro.112408.134123.View ArticlePubMedGoogle Scholar
- Cady KC, White AS, Hammond JH, Abendroth MD, Karthikeyan RS, Prajna L, Zegans ME, O'Toole GA: Prevalence, conservation and functional analysis of Yersinia and Escherichia CRISPR regions in clinical Pseudomonas aeruginosa Isolates. Microbiology. 157: 430-437. 10.1099/mic.0.045732-0.Google Scholar
- Labrie SJ, Samson JE, Moineau S: Bacteriophage resistance mechanisms. Nat Rev Microbiol. 2010, 8: 317-327. 10.1038/nrmicro2315.View ArticlePubMedGoogle Scholar
- Vitiello CL, Merril CR, Adhya S: An amino acid substitution in a capsid protein enhances phage survival in mouse circulatory system more than a 1000-fold. Virus Res. 2005, 114: 101-103. 10.1016/j.virusres.2005.05.014.View ArticlePubMedGoogle Scholar
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