Antigenic presentation of heterologous epitopes engineered into the outer surface-exposed helix 4 loop region of human papillomavirus L1 capsomeres
© Murata et al; licensee BioMed Central Ltd. 2009
Received: 03 April 2009
Accepted: 18 June 2009
Published: 18 June 2009
Human papillomavirus (HPV) L1 capsid proteins can self-assemble into pentamers (capsomeres) that are immunogenic and can elicit neutralizing antibodies. Structural modelling of L1 inter-pentameric interactions predicts that helix 4 (h4) of each of the five L1 monomers project laterally and outwards from the pentamer. We sought to utilize HPV L1 capsomeres as a vaccine platform by engineering heterologous epitopes within L1 derivatives deleted for h4 domain.
We used baculovirus – infected Trichoplusia ni cells and ultracentrifugation to synthesize and purify three 16L1 derivatives: one bearing a short deletion (amino acids 404–436) encompassing the h4 domain, and two others, each bearing a conserved neutralizing epitope of the human respiratory syncytial virus (RSV) fusion (F) protein (residues 255–278 and 423–436) that was substituted for the deleted L1 h4 domain residues. Each of the three capsomere derivatives was recognized by anti-L1 antibodies, while two bearing the RSV F-derived moieties were recognized by anti-RSV F antibodies. All three L1 derivatives formed ring-like structures that were similar in morphology and size to those described for native 16L1 capsomeres. When injected into mice, each of the capsomere derivatives was immunogenic with respect to L1 protein, and immunization with chimeric L1-RSV F pentamers resulted in RSV non-neutralizing antisera that recognized purified RSV F protein in immunoblots.
HPV L1 monomers bearing heterologous epitopes within the L1 h4 region can self-assemble into capsomeres that elicit antibody response against such non-HPV encoded epitopes. Thus, the L1 h4 region can function as a novel antigen display site within the L1 pentamer, which in turn may serve as a potential vaccine template.
Human papillomaviruses (HPVs) are non-enveloped DNA oncogenic viruses that cause significant burden of disease, including cervical dysplasia and cancer . The major structural component of the HPV virion is the L1 viral capsid protein that can spontaneously form pentamers (capsomeres) [2, 3]. Such L1 oligomers can then self-assemble into one of two virus-like particles (VLPs): a spherical lattice structure of T = 7 symmetry group that is morphologically indistinguishable from native HPV virions, or a smaller T = 1 particle that is comprised of 12 L1 pentamers and for which the crystal structure has been solved [3–5].
L1 VLP formation requires inter-capsomeric hydrophobic interactions involving helices 2, 3, and 4 (h2, h3, and h4, respectively) near the carboxy-terminus of each L1 monomer [3, 5, 6]. In L1 capsomeres, these helices project laterally and outwards onto the solvent-exposed surface. Helix 4 from a L1 monomer within a capsomere forms hydrophobic interactions with h2 and h3 of a L1 molecule of an adjacent capsomere to link the two L1 pentamers. Deletion of h4 has no obvious effect on L1 capsomere assembly, but abolishes the ability of L1 to form T = 1 or T = 7 VLPs .
In addition to its self-assembling capabilities, the papillomavirus L1 protein can function as potent immunogens when oligomerized as capsomeres and VLPs [4, 7, 8]. Bacterially derived L1 proteins from HPV type 16 (HPV-16) and other HPV serotypes as well as those derived from the oncogenic canine oral papillomavirus (COPV) form capsomeres in vitro and elicit neutralizing antibodies [9–12]. Immunization with COPV-L1 capsomeres generates a protective response in a subsequent COPV-canine oral mucosal challenge . The L1 HPV VLPs elicit robust neutralizing and protective antibodies, and have recently been licensed as prophylactic vaccines against HPV infection [13, 14].
The biophysical and immunological properties of HPV L1 capsomeres and VLPs suggest that these structures may function as vaccine platforms (reviewed in ). To this end, several studies have described the generation of chimeric VLPs bearing heterologous antigenic residues at the carboxy-terminus or surface-exposed loops of L1 monomers (e.g. [16–18]). However, the challenges of such approaches include inefficient antigen display, the limited structural capacity of L1 surface loops to accommodate foreign epitopes, and potentially significant disruption of L1 oligomeric structures. To circumvent these issues, we chose the L1 h4 domain as a novel antigen presentation site since this region is predicted to be surface-exposed in capsomeres. In place of the h4 and surrounding residues, we generated L1 derivatives bearing one of two previously characterized neutralizing epitopes of the RSV F protein . We demonstrate that L1 derivatives bearing either of the two foreign epitopes can form oligomers that are morphologically similar to capsomeres. Furthermore, such modified L1 pentamers can elicit antibodies that recognize the RSV F protein.
Expression and purification of HPV 16L1 derivatives bearing h4 deletion and substitutions
Immunological and structural characterization of L1 derivatives
To determine whether the capsomere derivatives bore the expected L1 and RSV F-derived epitopes, we subjected the capsomere preparations to a series of immunological tests. In ELISAs, control L1 VLPs and all three capsomeres were recognized by a mouse polyclonal anti-L1 VLP antiserum as well as anti-HPV 16L1 mAb V5, which recognizes an immunodominant epitope within 16L1 (Figure 2B and 2C) [20, 26]. Interestingly, as compared to L1 VLPs, all three capsomere derivatives were recognized more strongly by mAb V5. In immunoblots, the L1 derivatives were recognized by the CAMVIR-1 anti-HPV 16L1 mAb, with the C-1 deletion migrating slightly faster as anticipated and as compared to the L1 from VLPs or the 3-1 and 423-3 derivatives (Figure 2D). When tested for the presence of RSV F 255–278, the anti-RSV F mAb L4 recognized 3-1 but not C-1 or 423-3 in immunoblots (Figure 2D) or ELISAs (data not shown). The unavailability of an anti-RSV F mAb recognizing the second RSV F epitope (aa 423–436) within our antibody panel precluded similar analysis of 423-3. However, we were able to reproducibly detect the presence of 423-3 rabbit polyclonal anti-RSV F antisera (Figure 2D) . Thus we conclude that all capsomere derivatives in this study exhibit no gross deficiencies in L1-derived epitopes, and that two L1 derivatives express the RSV F epitopes as expected. In addition, the ELISA data using V5 mAb suggest that the V5 binding site may be more readily accessible in capsomeres than in VLPs and that deletions and substitutions of L1 h4 may subtly affect accessibility and/or conformation of the V5 recognition site.
Immunogenicity of HPV 16L1 derivatives
Because papillomavirus L1 protein-based oligomers (capsomeres and VLPs) can elicit a broad array of immune responses, they have been studied as potential vaccine platforms (reviewed in ). Such efforts have primarily focused on placing heterologous epitopes on surface-displayed, genetically variable loops of L1 or at the carboxy-terminus of full-length or truncated L1. Since capsomeres are also immunogenic, we tested the hypothesis that the h4 domain of the L1 monomer, which projects laterally and outwards on L1 pentamers, can be used for antigenic display of foreign epitopes. We demonstrate that: 1) such antigen presentation is feasible and does not overtly affect the formation of capsomeres; 2) such capsomeres likely exists as monomeric capsomeres with some degree of aggregation noted; and 3) mice immunized with capsomeres bearing RSV F epitopes generate antisera that recognizes the purified F protein. Thus, we conclude that foreign epitopes embedded within the h4 domain can be immunogenic when presented in the context of capsomeres.
Our findings have implications for structure of capsomeres. Two bacterially derived internal deletions spanning h4 – residues 410–427 and 404–436 that maintain or delete the h4-flanking prolines, respectively – can form capsomeres in vitro . Based on these results, we initially produced similar deletions for our experiments. Unexpectedly, we noted that multiple efforts to purify B-1 (lacking 413–430 and bearing h4 flanking prolines) and its derivatives bearing the two RSV F epitopes consistently yielded capsomere preparations of limited quality and quantity. There are several possible explanations for this observation, including: subtle differences in the sequences deleted in our experiments as compared to those used by others; the use of baculovirus-derived L1 instead of prokaryotically expressed proteins; and structural constraints induced by the juxtaposition of several proline residues following internal h4 deletion.
In contrast, the deletion of the entire h4 domain and the surrounding proline residues (C-1, aa 404–436) led to purification and enrichment of capsomere derivatives, which were used in subsequent experiments. Furthermore, this L1 derivative formed capsomeres in the presence of two different heterologous epitopes of varying length and predicted structures (RSV F 255–278: helix-coil-helix 24 aa vs. RSV F 423–436: linear 14 aa). Since h4 projects laterally and outwards from capsomeres, its location may be more permissive to aa insertions as compared to other regions of L1. However, we did note a limited degree of capsomere aggregation in EM and sucrose gradient analyses. Such inter-capsomeric interactions may be dependent on the size and the primary and secondary structures of the foreign epitope within the h4 domain. Aggregation of capsomeres, if any, did not appear to affect the immunogenicity with respect to L1 epitopes.
Immunization of mice with capsomeres bearing RSV F epitopes elicited anti-F antibodies as tested by immunoblots using purified F protein. However, these antisera did not recognize purified RSV F on ELISAs and were non-neutralizing (data not shown). Since bacterially derived RSV F 255–278 fused to the carboxy-terminus of cholera holotoxin was immunogenic and protective, it is possible that the embedded presentation of the epitopes within the h4 domain may have altered the anti-F antibody response. We also cannot exclude the possibility that capsomere aggregation influenced the anti-RSV F response or that the immune response against RSV F sequences may have been subdominant to those on L1 surface loops. Lastly, the antigen processing and presentation may have been affected by the use of Freund's adjuvants.
Our results serve as "proof-of-principle" studies to demonstrate that the h4 loop region of L1 can function as an antigen display site in the context of L1 capsomeres. Efforts to generate capsomere derivatives in prokaryotic systems, refine and improve the immunogenicity of modified L1 oligomers, and to expand the array of antigens for display, are in progress.
DNA constructions and manipulations
Plasmids and baculovirus stocks bearing HPV 16L1 cDNA have previously been described. Deletions of 16L1 cDNA were created by first ligating the entire cDNA of 16L1 into the BglII-SmaI site of pSP72 (Promega) to generate pSS1. Using PCR SuperMix High Fidelity (Invitrogen) and pSS1, inverse PCR was used to generate 16L1 derivatives deleted for aa 404–436 (del1) and aa 413–430 (del2). To enable modular oligonucleotide-based constructions, a unique NheI site encoding the residues ...AS... was engineered in place of each of the deletions. The following oligonucleotide 5' and 3' pairs (with the NheI site underlined) were used in PCR amplifications: del1: 5'GGCCGCTAGC AAAGAAGATCCCCTTAAAAAATATACTT; and 5'GGCCGCTAGC ATTCCAGTCCTCCAAAATAGT; del2: 5'GGCCGCTAGC CATACACCTCCAGCACCTAAAGAAGATC; and 5'GGCCGCTAGC GCCTCCTGGAGGAGGTTGTAAAC. The following PCR conditions were used: 94°C × 5 minutes × 1, then 35 cycles of 94°C × 1 minute, 55°C × 30 seconds, and 68°C × 5 minutes, followed by 68°C × 7 minutes and overnight storage at 4°C. The PCR amplicons were column purified (Qiagen), serially digested with NheI and DpnI (to remove the pSS1 template) and then self-ligated. The resulting plasmids were sequenced to confirm the existence of the respective deletions and NheI sites within the L1 cDNA. Thereafter, the modified L1 cDNAs were excised and ligated into the BglII-SmaI sites of pVL1392 (Orbigen), during which the NheI site remained unique within the resulting plasmids, termed pC-1 (bearing 16L1 del1) and pB-1 (bearing 16L1 del2).
To embed RSV F protein epitopes within the h4 domain of L1 derivatives, codon-optimized complementary oligonucleotides that encode aa 255–278 (SELLSLINDMPITNDQKKLMSNNV) and 423–438 (TASNKNRGIIKTFS) of RSV A2 strain F protein (GI: 333933) were used. These oligos bore NheI-compatible termini and were phosphorylated with T4 kinase (NEB), annealed, and ligated into the NheI site of pC-1 and pB-1. Sequencing of the resulting plasmids confirmed the existence of the appropriate oligonucleotide sequences and predicted to encode the aa sequence: AS...RSV F epitope...AS, in which the alanine and serine flanking the RSV-derived residues are derived from the NheI site. Note that the oligos were designed such that the first residue (S) of RSV F 255–278 starts from the serine incorporated from the NheI site, i.e. the amino terminus of the epitope-bearing sequence is: ...ASELL...
To construct the baculovirus stocks for expression of L1 proteins used in this study, pC-1 and pB-1 derivatives were co-transfected into Spodoptera frugiperda (Sf9; Invitrogen) cells with linearized baculovirus DNA (Baculo-Gold; BD Biosciences) and cellfectin (Invitrogen). After 72 hrs, the Sf9 serum-free media from each co-transfection was removed and the baculovirus stocks were serially propagated and plaque purified × 3 prior to use in T. ni cells.
Protein expression and purification
RSV F protein was purified as previously described. Infection of T. ni cells with baculovirus bearing the 16L1 cDNA and subsequent purification of L1 VLPs were performed as described. The purification of capsomere derivatives was based on previous protocols and is briefly described as follows. T.ni cells growing at log phase in 250 mL cultures (1 – 2 × 106 cells/mL) in serum free media (Express Five, Invitrogen) were infected with each of the appropriate baculovirus stocks at a multiplicity of infection (MOI) of ≥ 3. After 72 hrs, the cells were collected by centrifugation, resuspended in ice-cold PBS + Complete Protease Inhibitor cocktail (Roche), and lysed using a Dounce homogenizer × 20 strokes and and a sonicator (3 × 20–30 second bursts, continuous cycle, output 3–4). The resulting mixture was brought to 40% CsCl (Roche) in 1× PBS and subjected to isopycnic ultracentrifugation at 28,000 × rpm × 40 hrs at 4°C using a Beckman SW28.1Ti rotor. The visible L1 band within the CsCl gradient was removed, dialyzed against PBS/0.5 M NaCl for >1 hr, and then overlayed onto a 30%/63% sucrose cushion using PBS/0.5 M NaCl as the solvent. After centrifugation at 28,000 × rpm × 5 hr at 4°C in a SW28.1Ti rotor, the capsomere-enriched fraction at the 0%/30% sucrose interface was removed and dialyzed exhaustively against PBS/1 M NaCl prior to -80°C storage and subsequent analysis.
Immunological and structural characterizations of capsomere derivatives
For protein gel electrophoresis, capsomeres and L1 VLPs were mixed 1:1 with 2× SDS-sample buffer containing β-mercaptoethanol, heated at 95°C for 2–5 minutes, and then resolved on 10%/5% discontinuous SDS-PAGE using BioRad Protean Tetra-cell apparatus. Where appropriate, molecular weight markers (Novex and MagicMark; Invitrogen) were resolved in parallel and proteins were visualized by staining with Coomassie Brilliant Blue R-250. For immunoblots, the proteins were resolved on SDS-PAGE as above and then transferred onto nitrocellulose using a BioRad Trans-blot device (typically at 100V at room temperature for 1 hr). PBS + 0.1% Tween-20 (PBST) + 2% dried non-fat milk was used to block non-specific protein binding onto nitrocellulose. For detection of L1 protein, CAMVIR-1 (Santa Cruz Biotechnology) was used at 1:60,000 dilution followed by goat anti-mouse IgG heavy/light chain antibody-horseradish peroxidase (HRP) conjugate (Southern Biotech) at 1:20,000 in PBST. Antibody-antigen complexes were visualized by chemiluminescence (ECL; Pierce) and radiography (Kodak).
ELISAs were performed essentially as previously described. Typically, each protein for analysis was diluted with PBS and plated at 100 ng/well onto 96 well ELISA plates (Nunc) and incubated overnight at 4°C. Following incubation with primary antibodies as described in figure legends, alkaline phosphatase-conjugated goat anti-mouse secondary antibodies (Southern Biotech) and phosphatase substrate tablets (Sigma-Aldrich) were then used to visualize antigen-antibody complexes. The resulting colorimetric reactions were read at OD405 nm using a 96-well ELISA plate reader (Molecular Devices).
For electron microscopy analysis, capsomere samples (typically at 0.5–1 mg/ml in PBS/1 M NaCl) were diluted 1:10 in ice-cold PBS/1 M NaCl and then adsorbed onto carbon-coated grid for approximately 1 – 3 minutes. Excess fluid was then blotted with filter paper and the grids were negatively stained with 2% uranyl acetate. Images from grids were obtained using a Hitachi 7100 transmission electron microscope at 80 kV and 60,000 × – 100,000 × magnification. As control images for capsomeres, intact 16L1 VLPs were dissociated into capsomeres using previously described incubation conditions with dithiothreitol.
Sucrose gradients with standards (bovine catalase and E. coli β-galactosidase, Sigma-Aldrich; bovine serum albumin, VWR) were performed as described except that PBS/1 M NaCl was used as the sucrose solvent. Approximately 50 – 100 μg of each of the three standards were mixed with 100 μg of each of the capsomeres or intact L1 VLPs and subjected to ultracentrifugation at 41,000 × rpm × 16 – 20 hours at 4°C using a SW41.1Ti rotor (Beckman). The resulting gradient fractions (0.5 ml aliquots) were serially collected from the top of the ultracentrifuge tube, resolved on 10/5% SDS-PAGE, and then stained with Coomassie Brilliant Blue R-250 (for localization of standard peaks) or transferred to nitrocellulose and probed with CAMVIR-1 anti-L1 mAb prior to chemiluminescence detection.
Immunogenicity of capsomere derivatives in mice
The animals were fed standard diet and water ad libitum and housed a pathogen-free environment within the University of Rochester School of Medicine and Dentistry Vivarium. Prior to any immunogenicity studies, all animal care and use protocols used in this study were approved by the Institutional Animal Care and Use Committee at the University of Rochester Medical Center. Female 6 – 8 week old BALB/c mice (Jackson Laboratories) in groups of 4 – 5 mice per capsomere were injected intramuscularly with 50 ug of each of the capsomeres. For priming injection, the capsomeres were diluted 1:1 and emulsified with Freund's complete adjuvant, while for boosts at weeks 3 and 6, Freund's incomplete adjuvant was used at 1:1 dilution. At week 6, submandibular bleeds were performed on the mice and the resulting sera were analyzed for reactogenicity against purified RSV F protein and purifed 16L1 VLPs. For antisera against 16L1 VLPs, 50 μg of purified VLPs were injected intramuscularly into mice as above except that no adjuvants were used.
This work was supported by the NIH (R21 AI076781-02) as well as the 2007-8 Buswell Fellowship Award (Department of Medicine, University of Rochester School of Medicine and Dentristry) to YM. We thank Karen Bentley (University of Rochester Medical Center Electron Microscopy Core Director) for EM photographys and for helpful discussions.
- zur Hausen H, Gissmann L: Lymphotropic papovaviruses isolated from African green monkey and human cells. Med Microbiol Immunol (Berl) 1979,167(3):137-153. 10.1007/BF02121180View ArticleGoogle Scholar
- Rose RC, Bonnez W, Reichman RC, Garcea RL: Expression of human papillomavirus type 11 L1 protein in insect cells: in vivo and in vitro assembly of viruslike particles. J Virol 1993,67(4):1936-1944.PubMed CentralPubMedGoogle Scholar
- Modis Y, Trus BL, Harrison SC: Atomic model of the papillomavirus capsid. EMBO J 2002,21(18):4754-4762. 10.1093/emboj/cdf494PubMed CentralView ArticlePubMedGoogle Scholar
- Chen XS, Casini G, Harrison SC, Garcea RL: Papillomavirus capsid protein expression in Escherichia coli: purification and assembly of HPV11 and HPV16 L1. J Mol Biol 2001,307(1):173-182. 10.1006/jmbi.2000.4464View ArticlePubMedGoogle Scholar
- Chen XS, Garcea RL, Goldberg I, Casini G, Harrison SC: Structure of small virus-like particles assembled from the L1 protein of human papillomavirus 16. Mol Cell 2000,5(3):557-567. 10.1016/S1097-2765(00)80449-9View ArticlePubMedGoogle Scholar
- Bishop B, Dasgupta J, Chen XS: Structure-based engineering of papillomavirus major capsid l1: controlling particle assembly. Virol J 2007, 4: 3. 10.1186/1743-422X-4-3PubMed CentralView ArticlePubMedGoogle Scholar
- Rose RC, White WI, Li M, Suzich JA, Lane C, Garcea RL: Human papillomavirus type 11 recombinant L1 capsomeres induce virus-neutralizing antibodies. J Virol 1998,72(7):6151-6154.PubMed CentralPubMedGoogle Scholar
- Evans TG, Bonnez W, Rose RC, Koenig S, Demeter L, Suzich JA, O'Brien D, Campbell M, White WI, Balsley J, Reichman RC: A Phase 1 study of a recombinant viruslike particle vaccine against human papillomavirus type 11 in healthy adult volunteers. J Infect Dis 2001,183(10):1485-1493. 10.1086/320190View ArticlePubMedGoogle Scholar
- Rose RC, White WI, Li M, Suzich JA, Lane C, Garcea RL: Human papillomavirus type 11 recombinant L1 capsomeres induce virus-neutralizing antibodies. J Virol 1998,72(7):6151-6154.PubMed CentralPubMedGoogle Scholar
- Yuan H, Estes PA, Chen Y, Newsome J, Olcese VA, Garcea RL, Schlegel R: Immunization with a pentameric L1 fusion protein protects against papillomavirus infection. J Virol 2001,75(17):7848-7853. 10.1128/JVI.75.17.7848-7853.2001PubMed CentralView ArticlePubMedGoogle Scholar
- Fligge C, Giroglou T, Streeck RE, Sapp M: Induction of type-specific neutralizing antibodies by capsomeres of human papillomavirus type 33. Virology 2001,283(2):353-357. 10.1006/viro.2000.0875View ArticlePubMedGoogle Scholar
- Thones N, Herreiner A, Schadlich L, Piuko K, Muller M: A direct comparison of human papillomavirus type 16 L1 particles reveals a lower immunogenicity of capsomeres than viruslike particles with respect to the induced antibody response. J Virol 2008,82(11):5472-5485. 10.1128/JVI.02482-07PubMed CentralView ArticlePubMedGoogle Scholar
- Koutsky LA, Ault KA, Wheeler CM, Brown DR, Barr E, Alvarez FB, Chiacchierini LM, Jansen KU, Proof of Principle Study Investigators: A controlled trial of a human papillomavirus type 16 vaccine. N Engl J Med 2002,347(21):1645-1651. 10.1056/NEJMoa020586View ArticlePubMedGoogle Scholar
- Garland SM, Hernandez-Avila M, Wheeler CM, Perez G, Harper DM, Leodolter S, Tang GW, Ferris DG, Steben M, Bryan J, Taddeo FJ, Railkar R, Esser MT, Sings HL, Nelson M, Boslego J, Sattler C, Barr E, Koutsky LA, Females United to Unilaterally Reduce Endo/Ectocervical Disease (FUTURE) I Investigators: Quadrivalent vaccine against human papillomavirus to prevent anogenital diseases. N Engl J Med 2007,356(19):1928-1943. 10.1056/NEJMoa061760View ArticlePubMedGoogle Scholar
- Chackerian B: Virus-like particles: flexible platforms for vaccine development. Expert Rev Vaccines 2007,6(3):381-390. 10.1586/14760522.214.171.1241View ArticlePubMedGoogle Scholar
- Chackerian B, Lowy DR, Schiller JT: Induction of autoantibodies to mouse CCR5 with recombinant papillomavirus particles. Proc Natl Acad Sci USA 1999,96(5):2373-2378. 10.1073/pnas.96.5.2373PubMed CentralView ArticlePubMedGoogle Scholar
- Slupetzky K, Shafti-Keramat S, Lenz P, Brandt S, Grassauer A, Sara M, Kirnbauer R: Chimeric papillomavirus-like particles expressing a foreign epitope on capsid surface loops. J Gen Virol 2001,82(Pt 11):2799-2804.PubMed CentralView ArticlePubMedGoogle Scholar
- Varsani A, Williamson AL, de Villiers D, Becker I, Christensen ND, Rybicki EP: Chimeric human papillomavirus type 16 (HPV-16) L1 particles presenting the common neutralizing epitope for the L2 minor capsid protein of HPV-6 and HPV-16. J Virol 2003,77(15):8386-8393. 10.1128/JVI.77.15.8386-8393.2003PubMed CentralView ArticlePubMedGoogle Scholar
- Walsh EE, Hruska J: Monoclonal antibodies to respiratory syncytial virus proteins: identification of the fusion protein. J Virol 1983,47(1):171-177.PubMed CentralPubMedGoogle Scholar
- Rose RC, Bonnez W, Da Rin C, McCance DJ, Reichman RC: Serological differentiation of human papillomavirus types 11, 16 and 18 using recombinant virus-like particles. J Gen Virol 1994,75(Pt 9):2445-2449. 10.1099/0022-1317-75-9-2445View ArticlePubMedGoogle Scholar
- Lopez JA, Bustos R, Orvell C, Berois M, Arbiza J, Garcia-Barreno B, Melero JA: Antigenic structure of human respiratory syncytial virus fusion glycoprotein. J Virol 1998,72(8):6922-6928.PubMed CentralPubMedGoogle Scholar
- Toiron C, Lopez JA, Rivas G, Andreu D, Melero JA, Bruix M: Conformational studies of a short linear peptide corresponding to a major conserved neutralizing epitope of human respiratory syncytial virus fusion glycoprotein. Biopolymers 1996,39(4):537-548. PublisherFullText 10.1002/(SICI)1097-0282(199610)39:4<537::AID-BIP6>3.0.CO;2-YView ArticlePubMedGoogle Scholar
- Walsh EE, Cote PJ, Fernie BF, Schlesinger JJ, Brandriss MW: Analysis of the respiratory syncytial virus fusion protein using monoclonal and polyclonal antibodies. J Gen Virol 1986,67(Pt 3):505-513. 10.1099/0022-1317-67-3-505View ArticlePubMedGoogle Scholar
- Arbiza J, Taylor G, Lopez JA, Furze J, Wyld S, Whyte P, Stott EJ, Wertz G, Sullender W, Trudel M: Characterization of two antigenic sites recognized by neutralizing monoclonal antibodies directed against the fusion glycoprotein of human respiratory syncytial virus. J Gen Virol 1992,73(Pt 9):2225-2234. 10.1099/0022-1317-73-9-2225View ArticlePubMedGoogle Scholar
- Wu SJ, Schmidt A, Beil EJ, Day ND, Branigan PJ, Liu C, Gutshall LL, Palomo C, Furze J, Taylor G, Melero JA, Tsui P, Del Vecchio AM, Kruszynski M: Characterization of the epitope for anti-human respiratory syncytial virus F protein monoclonal antibody 101F using synthetic peptides and genetic approaches. J Gen Virol 2007,88(Pt 10):2719-2723. 10.1099/vir.0.82753-0View ArticlePubMedGoogle Scholar
- Ryding J, Dahlberg L, Wallen-Ohman M, Dillner J: Deletion of a major neutralizing epitope of human papillomavirus type 16 virus-like particles. J Gen Virol 2007,88(Pt 3):792-802. 10.1099/vir.0.82449-0View ArticlePubMedGoogle Scholar
- Li M, Cripe TP, Estes PA, Lyon MK, Rose RC, Garcea RL: Expression of the human papillomavirus type 11 L1 capsid protein in Escherichia coli: characterization of protein domains involved in DNA binding and capsid assembly. J Virol 1997,71(4):2988-2995.PubMed CentralPubMedGoogle Scholar
- McCarthy MP, White WI, Palmer-Hill F, Koenig S, Suzich JA: Quantitative disassembly and reassembly of human papillomavirus type 11 viruslike particles in vitro. J Virol 1998,72(1):32-41.PubMed CentralPubMedGoogle Scholar
- Rose RC, Bonnez W, Strike DG, Reichman RC: Expression of the full-length products of the human papillomavirus type 6b (HPV-6b) and HPV-11 L2 open reading frames by recombinant baculovirus, and antigenic comparisons with HPV-11 whole virus particles. J Gen Virol 1990,71(Pt 11):2725-2729. 10.1099/0022-1317-71-11-2725View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.