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Figure 2 | Virology Journal

Figure 2

From: Muscovy duck reovirus p10.8 protein localizes to the nucleus via a nonconventional nuclear localization signal

Figure 2

Transfection of DEF with different plasmids and amino acid sequence analysis of p10.8 protein. (A) Confluent monolayers of DEF cells were transfected with the pCDNA-p10.8 plasmid. The cells were fixed at 7 hpt, and then stained with serum against p10.8 and with DAPI. (B) Confluent monolayers of Vero cells were transfected with pEGFP/pEGFP-p10.8. The cells were fixed at 7 hpt and then stained with DAPI. Stained cells were visualized by use of fluorescence microscopy (magnification 400). (C) Deduced amino acid sequence of duck reovirus p10.8 protein. The basic putative NLS is underlined and the putative NES is shaded. Aromatic amino acids are in red.

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Virology Journal

ISSN: 1743-422X