Table 1 Primers and probes designed for RVB and RVC detection
Name | Nucleotide sequence (5’-3’) | Accession number of the referred strain and location of the primer/probe | Amplicon size |
|---|---|---|---|
RVB NSP5_16F | GTAGACGGCTGGAAACGTTG | KU562894.1(16–35) | 170 bp |
RVB NSP5_186R | GAATCAACCAARTCAAYATCAGATT | KU562894.1(186–162) | |
RVB NSP5_60P | ARTTGAACTCAGACGCTTCTGCCA | KU562894.1(81–60) | |
RVC VP6_322F | TTGTTAGAGAAGCTTCAAGAAATGG | MN206066.1 (321–345) | 146 bp |
RVC VP6_469R | ACAGGATTCTCACGTCTTGATTG | MN206066.1 (467–446) | |
RVC VP6_348P | ATGCAACCYCARTCRCCAGCYCTT | MN206066.1 (347–370) |