Table 1 Primers for characterization of the porcine RelA gene
Primer name | Primer Sequences 3’ to 5’ | Annealing temperature(°C) | Purpose | Product length |
|---|---|---|---|---|
P1 | F-TCAGACCTCTTCCCCCTCATCT R-GAGCTCACCTGTGGATGCAG | 58 | Polymorphism | 600bp |
P2 | F-AAAGAGGGGTTGTGAGCTTG R-CACCTCGATGTCCTCTGATG | 55 | Polymorphism | 464bp |
P3 | F-GGTGGCAGGGAACTGATTTA R-GACACAGATTTGCGTCCACT | 55 | Polymorphism | 826bp |
P4 | F-GCTGTTGTCCTCATGAACCT R-TGAGATTATGTCGCTTCGCC | 55 | Polymorphism | 764bp |
P5 | F-TCCAGCTGGGAAGAGCAA R-AGGAAGTCACCGGTTCCT | 55 | Polymorphism | 600bp |
P6 | F-TCTCCTCCAGCTCCCCA R-AGAAGGGCTGAGAAGTCCAT | 51 | Polymorphism | 620bp |