Fig. 3
From: A macrophage-cell model of HIV latency reveals the unusual importance of the bromodomain axis
Latency reversal in the established cell lines and MDMs using different latency-reversing agents. (A, B) Latency reversal was more efficient in dTHP-1 cells for all the LRAs that could reactivate HIV from latency. Ten different clones with latent HIV were reactivated with indicated LRAs for 48 h in uTHP-1 (A) or dTHP-1 cells (B). HIV reactivation from latency was measured with FACS of csGFP-expressing cells. (C) Latently infected MDMs can be reactivated from latency. MDMs from three healthy donors were infected with HIVGKO and sorted for latently infected mKO2-expressing cells. Cells were incubated with the indicated LRAs for 48 and reactivation was measured with csGFP FACS as before. Data are means and error bars indicate ± SEM (n = 3). *, p < 0.05, **, p < 0.001; ***, p < 0.0001, ns, not significant, Student’s t test