Fig. 7
Reactivity of anti-OROV mAb against different arboviruses. Huh 7.5 cell line (2 × 104 cells per well) was infected with DENV1 (MOI 2; A), DENV2 (MOI 2; B), DENV4 (MOI 2; C), ZIKV (MOI 2; D), MAYV (MOI 0.5; E) and OROV (MOI 0.01; F). After 48 h, the IFA assay was performed using anti-OROV 63B3E7 and 268B8A3 mAbs, anti-E protein of CHIKV that cross recognize MAYV (1G1) and anti-flavivirus family (4G2), followed by anti-mouse Ig conjugated to Alexa Fluor 488. In the images, MOCK represents uninfected cells after 48 h, and cells infected with respective virus for 48 h. As shows the merged image where in blue, nuclei stained with DAPI and, in green, OROV labeling through the mAbs was used, followed by anti-mouse IgG and IgM conjugated with Alexa Fluor 488 and in B, only stained by positive reaction with anti-mouse IgG and IgM conjugated with Alexa Fluor 488. The scale bar corresponds to 500 μm. Images are representative of one biological replica in triplicate