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Fig. 5 | Virology Journal

Fig. 5

From: Development of monoclonal antibodies against oropouche virus and its applicability to immunohistochemical diagnosis

Fig. 5

Reactivity of anti-OROV mAbs in different cell lines infected with OROV. The cell lines A549 (purple), Huh-7.5 (red), SH-SY5Y (blue) and Vero E6 (green) were infected with OROV (MOI of 0.01 for 48 h). After 48 h, the IFA assay was performed using anti-OROV 63B3E7 (A) and 268B8A3 (B) mAbs, followed by anti-mouse IgG and IgM conjugated to Alexa Fluor 488, respectively. The graphics show the normalized percentage of positive cells stained by each mAb in different cell lines infected by OROV. The graphics represent tree biological replica in triplicate

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Virology Journal

ISSN: 1743-422X