Fig. 4

Immunohistochemistry in mouse neuronal tissue infected by OROV. Black arrows show perinuclear staining in neuronal tissue of mice infected by OROV and stained with mAb 63B3E7 (A) and mAb 268B8A3 (C). Image depicting the absence of perinuclear tissue and neuronal cytoplasmic immunolabel of the mAb 63B3E7 (B) and mAb 268B8A3 (D) in a neuronal tissue of mice uninfected with OROV (MOCK). E–F, negative control immunohistochemistry in OROV-infected tissue with mAbs 4G2 (anti-flavivirus), and 1G1 (Anti-alphavirus), respectively. The scale bar corresponds to 25 μm