Fig. 3

Mass spectrometry analysis of the OROV protein showing electrophoretic mobility of 26 kDa. A. Coomassie-stained polyacrylamide gel showing the protein mass standard (M) and the partially purified OROV proteins. The gel band excised for mass spectrometry analysis is indicated by a dotted box. B. Amino acid sequence of the OROV nucleocapsid protein (Uniprot ID Q6XDT3) showing in blue the region identified by mass spectrometry. The red lines underneath the amino acid sequence indicate the delimitations of the 20 unique peptides identified by mass spectrometry. C. Parameters obtained from the mass spectrometry analysis of the OROV protein showing electrophoretic mobility of 26 kDa