Fig. 2

Western blots for the identification of the OROV protein target by the mAbs 63B3E7 and 268B8A3. OROV-infected Vero E6 cell purified (OROV) or MOCK reacted with anti-OROV mAbs 63B3E7 (A) and 268B8A3 (C). As controls an anti-OROV polyclonal ascitic fluid (B) and an anti-envelope of flavivirus mAb 4G2 (D). All were subjected to 13% SDS-PAGE and electroblotted onto nitrocellulose membranes. Proteins were stained with the mAbs, followed by an anti-mouse IgG or IgM conjugated to alkaline phosphatase. Molecular weight marker of 10–250 kDa