From: Identification and characterisation of Gamma-herpesviruses in zoo artiodactyla
FIRST-ROUND PCR | ||
---|---|---|
 | Forward | Reverse |
Degenerate primers | DFA: 5’-GAYTTYGCNAGYYTNTAYCC-3’ ILK: 5’-TCCTGGACAAGCARNYSGCNMTNAA-3’ | KG1: 5’-GTCTTGCTCACCAGNTCNCANCCYTT-3’ |
Deoxyinosine- Substituted equivalent primers | DFA: 5’-GAYTTYGCIaAGYYTIaTAYCC-3’ ILK: 5’-TCCTGGACAAGCARIaYSGCIaMTIaAA-3’ | KG1: 5’-GTCTTGCTCACCAGIaTCIaCAIaCCYTT-3’ |
Product 440Â bp | ||
SECOND-ROUND PCR | ||
 | Forward | Reverse |
Degenerate primers | TGV: 5’-TGTAACTCGGTGTAYGGNTTYACNGGNGT-3’ | IYG: 5’-CACAGAGTCCGTRTCNCCRTADAT-3’′ |
Deoxyinosine- Substituted equivalent primers | TGV: 5’-TGTAACTCGGTGTAYGGIaTTYACIaGGIaGT-3’ | IYG: 5’-CACAGAGTCCGTRTCIaCCRTAIaAT-3’ |
Product 220Â bp |