Fig. 1
From: Novel neutralizing SARS-CoV-2-specific mAbs offer detection of RBD linear epitopes
a Expression of RBD in BL21(DE3) in the presence of 0.1 mM IPTG for 3 h (lane 1), uninduced BL21(DE3) (lane 2), BL21(DE3) control in the absence of IPTG (lane 3), marker (M). b Fractions of rRBD purification using Ni column, c Purity assessment of RBD (10 µg) by SDS-PAGE, d Western blot analysis of rRBD using anti-His tag antibody, and e two SARS-CoV-2 vaccinated human serums; positive serums (lane1 and 2), negative serum (C)