Table 2 Thermal cycle protocol
From: Development of a highly sensitive and specific intact proviral DNA assay for HIV-1 subtype B and C
Cycling step | Temperature °C | Time | Ramp rate | #cycles |
|---|---|---|---|---|
Enzyme activation | 95 | 10 min | ~ 2 °C/sec | 1 |
Denaturation | 94 | 30 s | 40 | |
Annealing/extension | 53, 55, 58 or 60 | 1 min | ||
Enzyme deactivation | 98 | 10 min | 1 |