Fig. 2
From: Development of a highly sensitive and specific intact proviral DNA assay for HIV-1 subtype B and C
In silico analysis of the annotation of near-full length HIV-1 subtype B and subtype C sequences by the original and subtype B&C IPDA. 752 Subtype B and 697 subtype C sequences were analyzed and classified as intact, hypermutated, other defective and cis-acting sequences according to their annotation after sequencing. Frequency of these sequences predicted to be intact, defective, or inferred lack of signal by the IPDA are depicted. Because of large mismatches of the original IPDA primers and probes for intact subtype C sequences, no analyses were shown for defective subtype C sequences with the original IPDA. These details can be found in Additional file 1: Table S2