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Fig. 6 | Virology Journal

Fig. 6

From: Impaired influenza A virus replication by the host restriction factor SAMHD1 which inhibited by PA-mediated dephosphorylation of the host transcription factor IRF3

Fig. 6

Mechanism of H5N1 downregulation of SAMHD1 protein and RNA levels. A549 cells were infected with H5N1 at an MOI of 1.0 (A, B). A549 cells were treated with 0.1% dimethyl sulfoxide (DMSO), 10 μM MG132, 50 μM CQ and 160 μM 3-MA for 12 h prior to harvest. and subjected to IB at 48 h post infection (A) or at the indicated time points (B) with GAPDH as a loading control. Analysis of SAMHD1 promoter luciferase activity in 293T cells transfected with IRF3 and PA for 24 h. The results are expressed as the fold increase of luciferase activity in IRF3 and empty vector overexpression cells. The error bars represent data from three independent repeats (mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, paired t test)

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Virology Journal

ISSN: 1743-422X