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Fig. 4 | Virology Journal

Fig. 4

From: The artificial amino acid change in the sialic acid-binding domain of the hemagglutinin neuraminidase of newcastle disease virus increases its specificity to HCT 116 colorectal cancer cells and tumor suppression effect

Fig. 4

The construction of the S519G mutant rNDV virus. (A) Schematic diagram of the S519G mutant rNDV plasmid. The transgene cassette is composed of a GE-IG-GS sequence and a multi cloning site (MCS) in front of the N-terminus of the foreign gene insertion site, and can be constructed by inserting the transgene cassette between the NP and P genes, the P and M genes, the M and F genes, the F and HN genes, and the HN and L genes. (B) Growth kinetics of rNDV and S519G mutant rNDV viruses in Vero cells. The cells were respectively infected with rNDV and S519G mutant rNDV viruses at an MOI of 0.5. The supernatant was collected at 24, 48, 72, and 96 h post-infection for TCID50 titration on Vero cells in triplicates. The graph was showed in mean log10TCID50/mL with a deviation and error bars represent the standard deviation of the mean (n = 3). rNDV; recombinant NDV.

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Virology Journal

ISSN: 1743-422X