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Fig. 3 | Virology Journal

Fig. 3

From: The artificial amino acid change in the sialic acid-binding domain of the hemagglutinin neuraminidase of newcastle disease virus increases its specificity to HCT 116 colorectal cancer cells and tumor suppression effect

Fig. 3

Binding of the S519G mutant H-domain-EGFP protein to the cancer cell surface. (A) Schematic diagram of the S519G mutant H-domain-EGFP protein. The amino acid at positions 519 of the H domain were mutated from serine to glycine. The S519G mutant H-domain-EGFP protein was expressed and purified. (B) The purified S519G mutant H-domain-EGFP protein was analyzed by western blot. (C) The binding of the S519G mutant H-domain-EGFP protein on various cancer cells. The surface binding of the S519G mutant H-domain-EGFP protein on various cancer cells (HCT 116: colorectal cancer, T98G; glioblastoma, A549; lung cancer) and colorectal normal cells (CCD-18Co) of a S519G mutant H-domain-EGFP protein for various cancer cells using a fluorescence microscope. H domain, the globular head domain. EGFP; enhanced green fluorescent protein

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Virology Journal

ISSN: 1743-422X