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Fig. 2 | Virology Journal

Fig. 2

From: The artificial amino acid change in the sialic acid-binding domain of the hemagglutinin neuraminidase of newcastle disease virus increases its specificity to HCT 116 colorectal cancer cells and tumor suppression effect

Fig. 2

Binding of the S519G mutant H-domain-avidin protein to the cancer cell surface. (A) Schematic diagram of the S519G mutant H-domain-avidin protein. The amino acid at positions 519 of the H domain was mutated from serine to glycine. The S519G mutant H-domain-avidin protein were expressed and purified. (B) The protein was purified with ion exchange chromatography and analyzed by western blot. (C) The binding ability of the H-domain mutation-avidin protein. The protein was identified in colorectal cancer cells (HCT 116, SW620, HT29), colorectal normal cells (CCD18-Co), other cancer cells (A549; lung cancer, T98G; glioblastoma) and normal cells (MRC-5; lung normal cells). (n = 4, bar: SEM, *p < 0.05, **p < 0.01, ***p < 0.001 determined by the Student’s t-test). H domain, the globular head domain; M, HN mutation; C, control

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Virology Journal

ISSN: 1743-422X