Fig. 6

METTL3 knockdown inhibits JEV replication by enhancing the innate immune response. A Human interferon β was applied to neuro2a cells and the mRNA levels of IFN β, Mx1, and ISG15 were monitored at the selected time points. B Neuro2a cells were infected with JEV (MOI = 0.5), and the levels of IFN β, Mx1, and ISG15 mRNA were measured at the designated time points following the infection. C At 48 hpi, RT-PCR was conducted to measure the mRNA levels of IFN β, Mx1, and ISG15 in the METTL3 knockdown and control cells. D RT-PCR analysis was conducted to measure the relative JEV RNA level in JEV-infected METTL3 knockdown and control cells treated with or without ruxolitinib, at 48 hpi (n = 3). All data are the means ± SD of the indicated number of replicates. Statistical significance of the difference was determined by Student’ s unpaired t-test. *p < 0.05; **p < 0.01, *** p < 0.001; ns, nonsignificant