Fig. 5

m6A modification positively regulates the replication of JEV. A RT-PCR analysis was conducted on neuro2a cells which had been transfected with a specific siRNA and infected with JEV (MOI = 0.5) for 48 h, with three replicates, to measure the relative JEV RNA level. All data are the means ± SD of the indicated number of replicates. Statistical significance of the difference was determined by Student’s unpaired t-test. **** p < 0.0001. B-F Immunoblot analysis was conducted to measure NS3 expression in neuro2a cells that had been transfected with the indicated siRNA and infected with JEV (MOI = 0.5) for 48 h. G The PFU assay was performed twice to determine the titers of JEV in the supernatants of siMETTL3-treated samples at 48hpi. H-J RT-PCR and Immunoblot analyses were conducted to assess NS3 expression in neuro2a cells that had been overexpressing either METTL3 or YTHDF1, and then infected with JEV( MOI = 0.5) for 48 h