Fig. 4

JEV infection decreases the expression of METTL3 in vivo. A Neuro2a cells were infected with JEV(MOI = 0.5) at the indicated time, and the levels of m6A writers and readers in the RNA were subsequently assessed using RT-PCR. B The expression of m6A writers and readers was analyzed by immunoblotting with the indicated antibodies, and β-actin was used as a loading control. C C57BL/6 J mice aged 4—6 weeks were given a footpad injection of either JEV or PBS, and their brains were harvested at mild, moderate, and severe stages of the onset (n = 6).The mRNA level of m6A writers and readers was evaluated by RT-PCR. The data represent the change relative to the level of the PBS group. Data are shown as the mean ± SD. Three independent experiments were performed. Statistical significance was determined by Student’ s t-test. *p < 0.05. D The expression of m6A writers and readers was evaluated by immunoblotting with the indicated antibodies. β-actin was used as a loading control