Fig. 3
From: Repurposed drugs in combinations exert additive anti-chikungunya virus activity: an in-vitro study
Effect of combined repurposed drugs on CHIKV infection under posttreatment condition using IFA (A) Immunofluorescence assay images depict CHIKV-infected Vero CCL-81 treated with different concentrations of individual compounds under posttreatment conditions are presented where virus-infected cells are stained with FITC depicted in green, while cell nuclei, stained with DAPI, are depicted in blue. The merged image is also included for comprehensive visualization. 2FA 100µM, EM 200µM, EN 1.56µM, M 200µM (B) Immunofluorescence assay images of CHIKV infected Vero CCL-81 cells treated with different concentrations of combined compounds under post treatment conditions are presented. 2FA + EM 100µM + 200µM, 2FA + EM 50µM + 100µM, 2FA + EN 100µM + 1.56µM, 2FA + EN 50µM + 0.78µM, 2FA + M 100µM + 200µM, 2FA + M 50µM + 100µM. (C) The mean percentage of infected cells was calculated relative to the total number of cells. The values were expressed as mean ± standard error (SE) of triplicates. The virus-infected cells are shown in green and percent of infected cells is plotted on the graph