Fig. 1
From: Transcriptomic analysis reveals impact of gE/gI/TK deletions on host response to PRV infection
Sample assessment and RNA integrity analysis of the samples. (A) The deletion of PRV’s gE/gI/TK genes affected the viral copy number in cells under identical infection states. Biological replicates were performed in triplicate and technical replicates in duplicate; no viral nucleic acids were detected in the PK15 control group. (B) Each subplot shows the RNA electrophoresis graph and RIN value of a sample. The title of the subplot is the sample number, such as “PRV 2017 gEA/gI/TK01”. In the graph, the pink and green peaks represent 28 S and 18 S rRNA, respectively. The linear regression curve is used to calculate the RIN value, which indicates the level of RNA integrity. The higher the RIN value, the better the RNA quality. Generally speaking, RNA with a RIN value greater than 7 can be used for subsequent experiments