Fig. 9

Verification of interaction networks. a The transfection efficiency was observed by fluorescence microscopy. b Expression levels changes after miRNA mimics transfection. The overexpression effect of miRNA mimics in 293 T cells was used to detect miRNA and mRNA expression levels 48 h after transfection by RT-qPCR. One-way ANOVA with Dunnett’s multiple comparison test. Means ± SD, compared with the NC group. The experiments were performed at least three times independently. c Schematic drawing of the putative binding sites or mutations of miR-324-3p in LTF mRNA 3′ UTR. d Luciferase activity of each group was detected at 48 h post-transfection, compared with the NC group, *P < 0.05; ns, no significance