Fig. 1

Growth kinetics of octamer-mutated coronavirus and wt coronavirus at different multiplicities of infections in cultured cells. (A) The genome structure of wt MHV-59 (MHVwt) and MHV-A59 with octamer mutation (MHVoctm). (B)-(F) Growth kinetics of MHVwt and MHVoctm in ML cells infected with MOIs of 1, 0.1, 0.01, 0.001 or 0.0001. (G)-(K) The folds of virus titer of MHVwt over MHVoctm (wt/octm) at different times postinfection (8,16, and 24 hpi) with MOIs of 1, 0.1, 0.01, 0.001 or 0.0001 derived from Figures (B)-(F). (L)-(N) The folds of virus titer of MHVwt over MHVoctm (wt/octm) with different MOIs at 8 (L), 16 (M) and 24 (N) hpi derived from Figures (B)-(F). The values in (B)-(N) represent the mean ± standard deviation (SD) of three individual experiments. Statistical significance was evaluated using a t test: * P < 0.05, ** P < 0.01. MOI, multiplicity of infection; hpi, hours postinfection; MHVwt or wt, wild-type MHV; MHVoctm or octm, MHV with octamer mutation