Fig. 6
From: Cross protection to SARS-CoV-2 variants in hamsters with naturally-acquired immunity
Immunohistochemical detection of inflammatory cell infiltrate in infected hamster lungs infected with Ancestral and variants of concern. Infected hamster lung tissues were stained using anti-MPO (neutrophils), anti-IBA1 (macrophages) and anti-CD3 (T lymphocytes) antibodies. A Representative photomicrographs of MPO expression in neutrophils (dark brown) at 5 dpi are shown. B Representative photomicrographs of IBA1 expression in macrophages (dark brown) at 5 dpi are shown. C Representative photomicrographs of CD3 expression in T lymphocytes (dark brown) at 5 dpi are shown. By 5 dpi, neutrophils, macrophages and T lymphocytes were mainly found in consolidated areas. D Quantitative analysis of MPO, IBA1 and CD3 staining on IHC images from infected lungs at 2 and 5 dpi using Qupath software as described in Materials and Methods section. Data shown is mean ± SEM of 4 hamsters per group. * Indicates p value < 0.05; ** indicates p values < 0.01; *** indicates p values < 0.001