Fig. 2From: Comparison of the protective efficacy between single and combination of recombinant adenoviruses expressing complete and truncated glycoprotein, and nucleoprotein of the pathogenic street rabies virus in miceImmunofluorescence assay (IFA) and Western blot analysis for identification of G and N proteins expressed from recombinant adenoviruses. The IFA was performed in mock-infected 293A cells (a) and cells infected with recombinant adenoviruses Ad-0910 N (b), Ad-0910G (c), and Ad-0910Gsped (d). Cells were fixed 2 days post-infection and subjected to antibody staining using RABV G and N protein-specific antibodies. Specific fluorescence (arrows) was detected in 293A cells infected with each recombinant adenovirus. Bars, 100 μm. Western blot analysis was conducted using 293A cell lysates infected with Ad-0910G, Ad-0910Gsped and Ad-0910 N, and monoclonal antibodies against the G or N protein of RABV. (e) Lane 1, 293A cell lysates infected with Ad-0910G; lane 2, 293A cell lysates infected with Ad-0910Gsped; lanes 3 and 4, NG108–15 cell lysates infected with the ERA strain; lane 5, mock-infected 293A cell lysates, (f) lane 1, 293A cell lysates infected with Ad-0910 N; lane 2 NG108–15 cell lysates infected with the ERA strain; lane 3, mock-infected 293A cell lysatesBack to article page