Fig. 3From: Development and application of triple antibody sandwich enzyme-linked immunosorbent assays for begomovirus detection using monoclonal antibodies against Tomato yellow leaf curl Thailand virus Sensitivity analyses of TAS-ELISA using MAbs M1 and D2 for begomovirus detection. Sap extracts from TYLCTHV-infected tomatoes and SLCCNV-infected wax gourds were two-fold serially diluted in sap extracts from healthy tomatoes or healthy wax gourds from 1:40 to 1:5120 (w/v, g/ml) and used to determine the sensitivity of MAb M1- (a) or D2- (b) based TAS-ELISAs. Dashed lines indicate the cut-off values for the TAS-ELISAs (twice the OD405 of healthy controls)Back to article page