Fig. 3

Subcellular localization of Hsc70 protein in SSN-1 cells. a Sequence comparison of Hsc70 originated from human, mouse, and snakehead fish. CLUSTALW was used to align the amino acid sequences of Hsc70 (240-420 aa) from human (Homo sapiens), mouse (Mus musculus), and snakehead fish (Ophiocephalus argus Cantor). The nuclear localization signals (purple) and the nuclear export signals (yellow) are highlighted. b SSN-1 cells were transfected with the plasmid pEYFP-N1 or pEYFP–Hsc70. At 24 h post-transfection, cells were fixed with 4% paraformaldehyde, permeabilized with 0.2% Triton X-100 and incubated DAPI. Cells were imaged on a LSM510 Meta confocal laser-scanning microscope