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Fig. 2 | Virology Journal

Fig. 2

From: Subcellular localization and interactions of Infectious Salmon Anemia Virus (ISAV) M1 and NEP as well as host Hsc70

Fig. 2

Interaction between M1 and NEP protein detected by pull-down assay. The His-M1 protein (10 μg) was incubated with 10 μg of GST or GST-NEP. To detect His-M1 and GST-NEP, western blot analysis was performed using either anti-His (lower panel) or anti-GST (upper panel) antibodies. In this assay, 1/100 of the total volume of His-M1 protein was loaded as the input (lanes 1 and 2), and 1/10 of the total volume of each eluted sample was loaded to detect interacted proteins (lanes 3 and 4)

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Virology Journal

ISSN: 1743-422X