Fig. 1

Components of the Viral Ribonucleoprotein and Nucleoprotein. a Graphical illustration of the viral Ribonucleoprotein (vRNP) complex, comprised of the viral polymerase subunits, PB1, PB2, and PA, bound to both the 5′ and 3′ ends of the viral RNA segment, and multiple copies of nucleoprotein (NP). b Domains of Nucleoprotein monomer crystal structure [32] using Deep View-Swiss-PdbViewer 4.0, with head domain, body domain, RNA pocket, and tail loop regions labeled. c. Nucleoprotein body domain substitutions of NPbd3 using Deep View-Swiss-PdbViewer 4.0 to analyze the accessible residues in the body domain of the NP monomer crystal structure [32]. In both b and c, residue color represents accessibility within the NP monomer as determined by the Deep View-Swiss-PdbViewer 4.0 “color” tool, with greatest to least accessible as follows: red, orange, yellow, green, light blue, and dark blue. Residues 289, 293, 294, 308, and 309 mutated in NPbd3 are highlighted in C. d Nucleoprotein (NP) sequence alignment from select influenza subtypes using CLS freeware and amino acids 281-310. Colors correlate with amino acid property. Asterisks indicate NPbd3 mutant