Fig. 11

Western blot analysis of DENV 2 structural and non-structural proteins. HEK293T/17 cells were treated with the concentrations of orlistat as indicated or not treated (DENV) and subsequently mock infected (mock) or infected with DENV 2. At 24 h.p.i the expression of (a) DENV 2 E and NS3 proteins and (d) FASN was determined by western blotting. All filters were stripped and re-probed with actin as a loading control. Experiments were undertaken independently in triplicate and signals quantitated (b, c, e). Bars show mean +/−SD (*; p value <0.05)