Fig. 2
From: Interactions of human microglia cells with Japanese encephalitis virus
Chemokine responses of JEV-treated human microglia. Human M-MG were treated with (a upper panel) Alum, JEV vaccine (used at a concentration of 1.2 pg/cell), (a lower panel, b) Mock antigen, Nakayama and TC362 isolates (used at an MOI of 10 TCID50/cell otherwise indicated) at 37 °C for 24 h. Concentration of IL-1β and IFN-β as well as CCL2, CCL5, CXCL8, CXCL9 and CXCL10 were measured in supernatants. a Box plots for concentrations of the selected cytokines and chemokines. The black line represents the mean value; the error bars the standard deviation. b Curve line showing the effect of various doses of JEV on cytokines and chemokine ligands production. The marker represents the mean value and the error bar the standard deviation. Values are of 2 independent experiments with each condition performed in triplicate cultures. Asterisks show significant differences between Alum and JEV vaccine or between Mock and the indicated live JEV isolate, calculated with the student t-test (* : p < 0.05; ** : p < 0.01; *** : p < 0.001)