Table 1 Primer sequences used for flavivirus, yellow fever, West Nile, dengue consensus and sero type specific RT-PCR reactions
From: Detection of dengue virus serotypes 1, 2 and 3 in selected regions of Kenya: 2011–2014
Primer | Sequence | Base pair size of amplified product | Reference |
|---|---|---|---|
FU1 CFD3 | 5′- TAC AAC ATG ATG GGA AAG AGA GAG AA-3′ 5′- GTG TCC CAG CCG GCG GTG TCA TCA GC-3′ | 260 Flavivirus | [46] |
KP7 KP81 | 5′-GCA GAG TGA TCG ACA GCC G-3′ 5′-CCA CCA GAC CAT TCG GCA TG-3′ | 258 West Nile | [47] |
YF7 R CAGF | 5′- AAT GCT CCC TTT CCC AAA TA- 3′ 5′- CGA GTT GCT AGG CAA TAA ACA CAT TTG GA-3 | 670 Yellow Fever | [48] |
D1 D2 | 5′-TCAATATGCTGAAACGCGCGAGAAACCG-3′ 5′-TTGCACCAACAGTCAATGTCTTCAGGTTC-3′ | 511 Dengue consensus | [49] |
TS1 | 5′-CGTCTCAGTGATCCGGGGG-3′ | 482 (Dl and TS1) DENV1 | [49] |
TS2 | 5′-CGCCACAAGGGCCATGAACAG-3′ | 119 (Dl and TS2) DENV2 | [49] |
TS3 | 5′-TAACATCATCATGAGACAGAGC-3′ | 290 (Dl and TS3) DENV3 | [49] |
TS4 | 5′-CTCTGTTGTCTTAAACAAGAGA-3′ | 392 (Dl and TS4) DENV4 | [49] |