Fig 1

CV-A9 infection in the SW480 cell line is independent of an RGD and αV-integrins. a Fluorescence from flow cytometric analysis of integrin expression. SW480 cells were incubated with integrin specific monoclonal antibodies against β1, αVβ3 or αVβ6 (cell controls as shown in a white plot were incubated with a secondary antibody) and 10,000 cells were measured in each sample. The data was analyzed by the Cyflogic program and the area of positive signal is indicated in a white plot. In the case of αVβ3 the white area is shadowed by the negative dark background. Standard threshold values were used in the assays. b The ratio (SW480/A549) of integrin mRNA levels measured by quantitative PCR is indicated. c Immunofluorescence images of SW480 cells infected with the wild type CV-A9 and with the RGD-deletion mutant (CV-A9RGDdel). Antibodies specific to CV-A9 (green) were used. The nuclei (blue) were stained with Hoechst. The scale bar = 20 μm