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Fig. 4 | Virology Journal

Fig. 4

From: Classical swine fever virus NS5A protein changed inflammatory cytokine secretion in porcine alveolar macrophages by inhibiting the NF-κB signaling pathway

Fig. 4

Effects of CSFV NS5A on the protein expression and nuclear translocation of NF-κB. Cells were treated as demonstrated in Fig. 2. Expression of NF-κB in PAMs was measured by Western Blot with antibodies specific for NF-κB (a). Cells were fixed and the localization of NF-κB (red) was observed by fluorescence microscope using immunofluorescence stain with anti-NF-κB/p65 and QDs-SA 605-conjugated biotinylated secondary antibodies. Nuclei were stained with DAPI. Bar, 10 μm (b). NF-κB Luciferase reporter assay was done to determine NF-κB luciferase activities (c). Representative results are shown of one of three separate experiments. An asterisk indicates a statistically significant difference from uninfected cells, *P < 0.05 and **P < 0.01

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Virology Journal

ISSN: 1743-422X